| Abstract: | objective: To construct the bone morphogenetic protein (BMP) single chain Fv (scFv) and obtain its expression. Methods: Using a synthesized peptide linker containing 15 amino acids, the N end of the heavy chain gene fragment of a strain of murine anti-BMP McAb was connected with the C end of its light chain gene fragment with subcloning. Then the recombinant BMPscFv was cloned into pGEX-4T-1 plasmid and in duced to express in E. coli JM 109. Results: The full length of the recombinant BMPscFv gene was 705 bp and its fusion protein was about 52 kD. Conclusion: Subcloning is a rapid, simple and reliable method for construction of scFv. |
