The relationship between epithelial Ia expression and the inflammatory cell infiltrate during experimental oral carcinogenesis |
| |
Authors: | J B Matthews A Pitigala-Arachchi I J Crane C Scully S S Prime |
| |
Institution: | (1) Department of Oral Pathology, The Dental School, University of Birmingham, St Chads Queensway, BY 6NN Birmingham, UK;(2) Department of Oral Medicine, Surgery and Pathology, University of Bristol, UK |
| |
Abstract: | Summary The development of oral epithelial expression of Ia antigens and its relationship to the presence of IL-2r+ (CD25+) cells was investigated in rats treated with the water soluble carcinogen 4-nitroquinoline-N-oxide (4NQO). Acetone fixed frozen sections of the palate and tongue were stained using an indirect immunoperoxidase technique and monoclonal antibodies to rat Ia (I-A & I-E) and IL-2 receptor. After 4 weeks 4NQO treatment all rats expressed oral epithelial Ia but thereafter (2–9 months) expression was present in only 20–40% of animals. Epithelial expression of Ia by histologically normal, dysplastic and neoplastic epithelium was always associated with the presence of an underlying inflammatory cell infiltrate containing CD25+ cells. Overall there were significantly more CD25+ cells in tissue specimens containing Ia+ epithelium compared with Ia– epithelium. Furthermore, during the first 4 weeks of carcinogen treatment, a significant positive correlation was found between the CD25+ cell density and occurrence of focal epithelial Ia expression. These results, together with analysis of the T cell, NK cell, macrophage and B cell content of the infiltrates induced by 4NQO, suggest that the CD25+ cells represent activated T cells. Thus, our results in this experimental model are consistent with the idea that epithelial expression of Ia is the result of production of IFN- by locally activated T cells. |
| |
Keywords: | Carcinogenesis Epithelium Ia antigens Il-2 receptor Inflammatory cells |
本文献已被 SpringerLink 等数据库收录! |
|