Electron spin resonance studies on the oxidative denaturation of hemoglobin of blood preserved at 20 degrees C]

In order to further understand the injurious change of erythrocytes during blood preservation at room temperature, we studied the denaturation of oxidative hemoglobin of blood preserved at 20 degrees C using electron spin resonance (ESR) technique. The results showed: when blood was preserved at 20 degrees C, there was ESR absorption of low spin hemichrome (g = 2) during the 204-hour preservation period; when the blood was preserved at 4 degrees C after it had been preserved at 20 degrees C for 24 hours, there were ESR absorption of the high spin methemoglobin (g = 6) and ESR absorption of low spin hemichrome (g = 2) during the 40-day preservation period. This study demonstrates that ESR absorption will increase when preservation time increases. When blood is preserved at 4 degrees C, no ESR absorption will emerge during 40 days' preservation.