多效生长因子在牙周炎治疗前后的表达情况

目的研究多效生长因子(pleiotropin,PTN)在牙周炎治疗前后的表达情况。方法应用RT-PCR、原位杂交方法,利用雄性Wistar大鼠50只分为炎症组、恢复组和对照组分别检测PTN的表达情况。结果 RT-PCR半定量分析PTNmRNA在炎症组和恢复组两组中的表达P<0.01,有统计学意义的。PTNmRNA的阳性产物位于牙龈上皮、龈沟内上皮和结合上皮细胞胞浆中,呈棕黄色颗粒。炎症组中PTNmRNA的表达主要集中于棘细胞层和颗粒细胞层,表达量为弱阳性和中强阳性。恢复组中PTNmRNA的表达主要集中于棘细胞层和基底细胞层,表达量为强阳性和中强阳性。对照组中PTNmRNA的表达较少。RT-PCR与原位杂交检测结果基本一致(P<0.05)。结论 PTN同时具有致炎和促进组织修复的功能,但促进组织修复的作用更明显。

The pleiotrophin expression in periodontitis before and after treatment

Objective To study the pleiotrophin expression in periodontitis before and after treatment.Methods RT-PCR and in situ hybridization analysis for PTN mRNA were performed on 50 male Wistar mice which wered divided into inflammation group,recovered group and control group.Results RT-PCR half-quantity analysis： There were obvious statistical significance between the inflammation group and the recovered group（P0.01）.The positive products（-brown-yellow grains） of PTN lied in the cytoplasm of gingival epithelium,gingival sulcus epithelium and junctional epithelium cells and occasionally in the nucleus of cells.Positive products expressed major in the spinous cell layer and granular cell layer in the inflammation group,and the expressions were weak-strong and mid-strong positive.Positive products expressed major in the spinous cell layer and basilar cell layer in the recovered group,and the expressions were mid-strong and strong positive.The expression was less in the control group.The result of RT-PCR was same to the result of in situ hybridization（P0.05）.Conclusion PTN have the functions of bringing about inflammation and promoting the injured tissue to recover.The function of recovery was more remarkable.