树鼩茄病镰刀菌性角膜炎房水中Th1/Th2炎性因子的变化

目的 观察树鼩茄病镰刀菌性角膜炎房水中Th1/Th2炎性因子的变化,了解Th1/Th2炎性因子与本病炎症反应的关系.方法 茄病镰刀菌培养7d后收集真菌混悬液,调整孢子密度为10×109 CFU·mL-1.清洁级树鼩40只随机分为实验组30只、对照组10只,右眼为实验眼.实验组将真菌孢子混悬液50 μL注入角膜基质中央,对照组注入生理盐水50 μL.造模后第3天、第7天、第14天,流式细胞仪分析房水中白细胞介素(interleukin,IL)-1β、IL-6、IL-4、IL-10的水平;病理学检查观察浸润细胞类型.结果 Th1型细胞因子IL-1β和IL-6浓度均在造模后第7天达到高峰,造模后各时间点与对照组相比差异均具有统计学意义(均为P＜0.05);Th2细胞因子IL-10浓度在造模后第14天达到高峰,造模后第7天、第14天与对照组相比差异均具有统计学意义(均为P ＜0.05);IL-4浓度仅在造模后第7天与对照组相比差异有统计学意义(P＜0.05).病理学检查结果示:炎症细胞浸润数量造模后第7天达到高峰,以中性粒细胞为主;实验组各时间点均可见菌丝平行于基质纤维生长.结论 促炎因子IL-β和IL-6及抑炎因子IL-10在树鼩茄病镰刀菌性角膜炎炎症反应机制中发挥重要作用.

Changes of Th1/Th2 inflammatory factors in the aqueous humor of tree shrews with fusarium solani keratitis

Objective To observe the changes of Th1 / Th2 inflammatory factors in the aqueous humor of tree shrews with fusarium solani keratitis,as well as to explore the relationship between Thl / Th2 inflammatory factors and inflammatory response in fusarium solani keratitis.Methods Forty healthy tree shrews were randomly divided into experimental group(n =30) and control group (n =10).Fusarium solani was inoculated into sabina culture medium and cultured at 26 ℃ for 7 days,and then the fungal suspension was collected and the density of spores was adjusted to 10 × 109 CFU · mL-1.In the experimental group,50 μL fungal spore suspension was injected into the center of the cornea stroma,while the control group received the same amount of saline.Next the levels of cytokines including interleukin (IL)-1 β,IL-6,IL-4 and IL-10 were analyzed by flow cytometry on day 3,day 7,day 14 after successful modeling,and the changes in types of infiltrating cells were observed by histopathological examination.Results The expression level of IL-1 β and IL-6 (Th1 type cytokines) was the highest on day 7,and the difference was statistically significant at each time point when compared with the control group (all P ＜ 0.05).The expression level of IL-10 (Th2 type cytokines) was the highest on day 14,and the difference was statistically significant at each time point when compared with the control group (all P ＜ 0.05).The difference in IL-4 expression was statistically significant on day 7 (P ＜ 0.05).In addition,histopathological examination showed that the number of infiltration cell reached its peak on day 7,mainly neutrophils,and fungal hyphae were observed to be parallel to the matrix fibers at each time point.Conclusion The proinflammatory cytokines IL-1β and IL-6 and the anti-inflammatory cytokines IL-10 may play an important role in the molecular mechanism of inflammatory response of fusarium solani keratitis in tree shrews.