BMT-1抑制急性B淋巴母细胞瘤细胞增殖活力及其机制研究

目的 探讨苯并咪唑衍生物BMT-1对急性B淋巴母细胞瘤细胞增殖活力的影响及其相关机制.方法 采用CCK-8方法测定BMT-1对Daudi、Nalm-6的细胞毒性;采用流式细胞技术方法检测BMT-1对Daudi细胞凋亡及线粒体膜电位的影响;采用Western Blot方法检测Daudi细胞凋亡相关蛋白PARP的表达情况.结果BMT-1对Daudi、Nalm-6细胞具有较强的细胞毒性;BMT-1可诱导Daudi细胞凋亡,引起Daudi细胞线粒体膜电位下降,并可使得Daudi细胞产生PARP蛋白剪切体.结论 BMT-1对B淋巴母细胞瘤具有细胞毒性作用,其关键机制为诱导细胞凋亡和引起线粒体膜电位下降,为BMT-1治疗急性淋巴细胞白血病提供了理论基础.

The Inhibition Effect of BMT-1 on the Proliferation of Acute B Lymphoblastoma Cells and Its Mechanism

Objective To investigate the effect of benzimidazole derivative BMT-1 on the proliferation of Acute B lymphoblastoma cells and its related mechanism.Methods The cytotoxicity of BMT-1 to Daudi and Nalm-6 was determined by the CCK-8 method.The effect of BMT-1 on the apoptosis and mitochondrial membrane potential of Daudi cells was detected by the flow cytometry.The expression of the apoptosis-related protein PARP in Daudi cells was detected by Western blot.Results BMT-1 was cytotoxic to the cells of Daudi and Nalm-6.BMT-1 induced the apoptosis of Daudi cells.Meanwhile,BMT-1 could reduce the mitochondrial membrane potential of Daudi cells and make Daudi cells produce the cleaved protein of PARP.Conclusion BMT-1 has a significant cytotoxic effect on B lymphoblastoma,and its key mechanism is to induce the apoptosis and the decrease of mitochondrial membrane potential,which provides a theoretical basis for the treatment of acute lymphoblastic leukemia with benzimidazole derivatives.