鼠李糖乳杆菌对过敏性鼻炎小鼠模型的免疫调节作用

目的 探讨鼠李糖乳杆菌(lactobacillus rhamnosus GG,LGG)对卵清蛋白(ovalbumin,OVA)诱导过敏性鼻炎(allergic rhinitis,AR)小鼠模型的免疫调节作用. 方法 6~8周龄BALB/c小鼠,随机分为阴性对照组、LGG组及AR组,每组各10只.采用Luminex法检测各组血清IL-4 、IL-10和IFN-γ水平.ELISA测血清OVA特异性IgE(OVA-sIgE)水平,采用流式细胞术检测脾细胞悬液中CD4+CD25+ T细胞比例.结果 与AR组比较,LGG可明显降低血清IL-4、IL-10及OVA-SIgE水平(P<0.05),提高脾细胞悬液中CD4+CD25+ T细胞比例( P<0.05);LGG组血清IFN-γ水平与AR组比较,差异无统计学意义(P=0.215).结论 LGG能通过抑制Th2细胞反应及增强CD4+CD25+ T细胞活性发挥抗过敏作用.

Immunomodulatory Effect of Lactobacillus Rhamnosus GG on the BALB/c Mice with Allergic Rhinitis

Objective To investigate the immunomodulatory effect of Lactobacillus rhamnosus GG (LGG) on the BALB/c mice with allergic rhinitis (AR) induced by ovalbumin (OVA).Methods The BALB/c mice aged from 6 to 8 weeks were divided randomly into the control group, LGG group and AR group, and each group consisted of 10 mice.The Luminex method was used to detect the levels of IL-4, IL-10 and IFN-γ in each group, the ELISA method was adopted to detect the level of OVA-specific IgE (OVA-sIgE), and the flow cytometry assay was used to examine the splenic percentage of CD4+CD25+Tregs.Results Compared with the AR group, LGG significantly reduced the levels of serum IL-4, IL-10 and OVA-specific IgE respectively (P<0.05) and increased the splenic percentage of the CD4+CD25+Tregs (P<0.05).There were no significant differences in the level of IFN-γ between the LGG group and the AR group (P=0.215).Conclusions LGG exerts the anti-allergic effect by inhibiting the Th2 immune response and inducting the activity of CD4+CD25+Tregs.