Identification of differentially expressed genes in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis |
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| Authors: | Cui Xiao-Nan Tang Jian-Wu Hou Li Song Bo Ban Li-Ying |
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| Affiliation: | 1. Department of Oncology, The 1st Affiliated Hospital of Dalian Medical University, Dalian 116011,Liaoning Province, China
2. Department of Pathology,Dalian Medical University, Dalian 116027, Liaoning Province,China |
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| Abstract: | AIM: To identify genes differentially expressed in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis. METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with low potential of lympho- genous metastasis Hca-P and its synogenetic cell line Hca-F with high metastatic potential was constructed by suppression subtracted hybridization (SSH) method. The screened clones of the subtracted library were sequenced and GenBank homology search was performed. RESULTS: Fifteen differentially expressed cDNA fragments of Hca-P were obtained which revealed 8 known genes, 4 expressed sequence tags (ESTs) and 3 cDNAs showed no homology. CONCLUSION: Tumor metastasis is an incident involving multiple genes. SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes. |
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| Keywords: | Suppression subtracted hybridization Liver neoplasm Metastasis suppression genes |
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