兔眼脉络膜上腔出血模型建立及自然转归的研究

目的建立兔眼脉络膜上腔出血（SCH）模型，观察其自然转归。方法经隧道式巩膜全层切口注入兔眼脉络膜上腔自体抗凝血建立SCH模型，并行检眼镜、B型超声及组织病理学观察。结果术后实验组兔眼均出现了SCH。术后1h视网膜及脉络膜呈明显红色隆起，边界清楚，出血范围为8～10个视盘直径；术后1d出血范围扩大、隆起度降低，术后3d出血开始吸收，术后7d大部分吸收，术后14d基本吸收，术后21d完全吸收。术后1～3d脉络膜上腔炎性细胞浸润，术后7d脉络膜上腔血液部分溶血，术后14d完全溶血，光感受器层发生空泡变性，睫状体萎缩。结论经隧道式巩膜全层切口注入兔眼脉络膜上腔自体抗凝血建立SCH模型的方法简单实用、安全可靠、重复性较好，为视网膜受损及相关治疗提供依据。

Study on establishment of suprachoroidal hemorrhage model and its natural course in rabbit

Objective Suprachoroidal hemorrhage has a worse prognosis in clinic and the surgery outcome is not ideal generally. This study was designed to investigate the method and safety of the establishment of suprachoroidal hemorrhage model in rabbit and observe its natural course. Methods 0. 1 mL auto-anticoagulation blood was injected into the suprachoroidal space via the full-thick scleral tunnel incision under the operation microscope to establish the suprachoroidal hemorrhage model in 12 adult healthy rabbits. Two normal rabbits were as control group. The clinical examinations including direct and indirect ophthalmoscopy, fondus photography, B-scan ultrasonography were performed at 1 hour, 1 day,3 days,7 days, 14 days and 21 days after operation. The histopathologic examination was performed at above time points under the light microscope. Results The clinical examination demonstrated that supraehoroidal hemorrhage models were successfully established in all the 12 rabbits without the evidence of anterior segment damage. Retinas and choroids of model rabbits were obviously eminenced 1 hour after operation with the range of 8 - 10 disc diameter （DD） and well-defined border. The size of suprachoroidal hemorrhage extended and degree of eminence decreased I day later. The suprachoroidal hemorrhage began to be absorbed at 3 days and mostly absorbed 7 days later and completely disappeared at 21 days after operation. The inflammatory cell infiltration was seen in the suprachoroidal space during the period of 1 to 3 days after operation, and the blood was hemolyzed incompletely 7 days after operation. Vaeuolation degeneration in photoreceptor layer was seen on 14 days and became more obvious on 21 days after operation. The atrophy of ciliary processes on 14 days and became severer on 21 days after operation. Conclusion Establishment of suprachoroidal hemorrhage model mentioned in this paper is a simple, safe and reproducible way. This study result offer a reference for the management of retinal damage induced by suprach