Bcl-2 inhibits the caspase-dependent apoptosis induced by SARS-CoV without affecting virus replication kinetics |
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| Authors: | L Bordi C Castilletti L Falasca F Ciccosanti S Calcaterra G Rozera A Di Caro S Zaniratti A Rinaldi G Ippolito M Piacentini M R Capobianchi |
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| Institution: | (1) Laboratory of Virology, National Institute for Infectious Diseases INMI “L. Spallanzani”, Rome, Italy;(2) Laboratory of Cell Biology and Electron Microscopy, National Institute for Infectious Diseases INMI “L. Spallanzani”, Rome, Italy;(3) Department of Epidemiology, National Institute for Infectious Diseases INMI “L. Spallanzani”, Rome, Italy;(4) Department of Biology, University of Rome “Tor Vergata”, Rome, Italy |
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| Abstract: | Summary. Vero cells transfected with either neo- or bcl-2-plasmid were infected with SARS-CoV at a high multiplicity of infection. Apoptosis appeared after the onset of CPE and completion
of virus replication, and could be prevented by Bcl-2 expression. Apoptosis is likely mediated by the mitochondrial pathway,
as demonstrated by its inhibition using Bcl-2, and by the activation of the caspase cascade, resulting in PARP cleavage. Prevention
of apoptosis did not affect susceptibility to infection, kinetics and extent of viral replication and release, thus implying
that apoptosis is not involved in facilitating release and/or dissemination of SARS-CoV in Vero cells. |
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