婴儿肝病综合征患儿外周血有核细胞Toll样受体的表达

目的:探讨婴儿肝病综合征患儿外周血有核细胞Toll样受体(TLR s)的表达。方法:婴儿肝病综合征患儿25例,正常对照组12例。采用RT-PCR法检测外周血有核细胞TLR 4、TLR 2 mRNA的变化,采用ELISA法检测血清TNFα、IL-6水平,同时检测内毒素水平,并分析TLR 4、TLR 2 mRNA水平与血清总胆红素(TB)、直接胆红素(DB)的关系。结果:婴儿肝病综合征患儿TLR 4、TLR 2 mRNA的表达及TNFα、IL-6水平明显高于正常对照组,差异有极显著性意义(P<0.01)。两组内毒素水平无明显差异(P>0.05)。TLR 4、TLR 2 mRNA的表达与血清TB、DB无相关关系(P>0.05)。结论:婴儿肝病综合征患儿体内TLR s的表达增强,提示TLR s在婴儿肝病综合征发病机理中起作用。

The expression of toll-like receptors in the peripheral white blood cells of the infants with infantile hepatitis syndrome

Objective:To explore the expression of tolllike receptors in the peripheral white blood cells of the infants with infantile hepatitis syndrome.Methods:The study was done in 25 infants with infantile hepatitis syndrome.12 healthy infants were as control.The expression of TLR4,TLR2 mRNA in the peripheral white blood cells was observed by RT-PCR,the level of serum TNFα and IL-6 was assayed by ELISA and the level of endotoxin was detected.The correlation between TLR4,TLR2 mRNA and total bilirubin(TB),direct bilirubin(DB)was analyzed.Results:Compared with the control group,the expression of TLR4,TLR2 mRNA and the level of TNFα,IL-6 were significantly elevated(P<0.01) in the infants with infantile hepatitis syndrome.There was no significant difference in the level of endotoxin between the two groups(P>0.05).There was no correlation between TLR4,TLR2 mRNA and TB,DB.Conclusion:The expression of toll-like receptors in the infants with infantile hepatitis syndrome is elevated,suggesting that TLRs play a role in the pathogenesis of infantile hepatitis syndrome.