| 大鼠外周血内皮祖细胞体外向内皮细胞诱导分化时的表型变化特征 |
| |
| 引用本文： | 池一凡,孙龙,侯文明,孙忠东,牛兆倬,孙勇. 大鼠外周血内皮祖细胞体外向内皮细胞诱导分化时的表型变化特征[J]. 青岛大学医学院学报, 2008, 44(4) |
| |
| 作者姓名： | 池一凡 孙龙 侯文明 孙忠东 牛兆倬 孙勇 |
| |
| 作者单位： | 青岛市市立医院心脏外科,山东,青岛,266011 |
| |
| 摘 要： | 目的 研究大鼠外周血内皮祖细胞(EPC)体外分化为内皮细胞的过程中,干细胞标志物以及内皮细胞表型的变化.方法 取SD大鼠外周血,应用Ficoll密度梯度离心的方法获得单个核细胞,体外培养于纤连蛋白处理的培养皿中,同时应用VEGF和bFGF诱导,使之向内皮细胞转化,用免疫组织化学方法检测细胞CD31、CD34、Flk-1、vWF在第1～7天的表达.结果 贴壁细胞的CD31、CD34、Flk-1、vWF在不同时段呈不同程度的表达.干细胞的标志物CD31、CD34的表达逐步减弱,第6天时消失.内皮标志物Flk-1和 vWF表达逐步增强,第7天时达100%.结论 大鼠外周血干细胞向内皮祖细胞分化的过程中,干细胞标志物逐渐消失,内皮细胞的表型逐步显现.
|
| 关 键 词： | 内皮祖细胞 内皮细胞 细胞分化 大鼠 |
IN VITRO DIFFERENTIATION OF ENDOTHELIAL PROGENITOR CELL FROM RAT PERIPHERAL BLOOD |
| |
| CHI YI-FAN,SUN LONG,HOU WEN-MING,et al. IN VITRO DIFFERENTIATION OF ENDOTHELIAL PROGENITOR CELL FROM RAT PERIPHERAL BLOOD[J]. Acta Academiae Medicinae Qingdao Universitatis, 2008, 44(4) |
| |
| Authors: | CHI YI-FAN SUN LONG HOU WEN-MING et al |
| |
| Affiliation: | CHI YI-FAN,SUN LONG,HOU WEN-MING,et al (Department of Cardiac Surgery,Qingdao Municipal Hospital,Qingdao 266011,China) |
| |
| Abstract: | Objective To study the changes of surface markers of endothelial progenitor cell during differntiation into endothelial cells. Methods Mononuclear blood cells(PBMC) were isolated from rat peripheral blood by means of Ficoll density gradient centrifugation and cultured in the presence of VEGF and bFGF.Expressions of CD31,CD34,Flk-1 and vWF on adherent cells were assayed with immunochemistry technique. Results The expressions of CD31,CD34,Flk-1 and vWF on adherent cells changes during the culture peroid.The p... |
| |
| Keywords: | Endothelial progenitor cell Endothelial cells Cell differentiation Rats |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
