原肌球蛋白、丙酮酸激酶在急性早幼粒细胞白血病耐药细胞中表达上调

目的应用蛋白质组学方法筛选人急性早幼粒细胞白血病（APL）维甲酸敏感细胞株（NB4细胞株）和维甲酸耐药细胞株（NB4-R1细胞株）的耐药相关蛋白。方法利用双向凝胶电泳技术检测，比较NB4和NB4-R1细胞株的总蛋白，建立二维凝胶电泳（two-dimensional gel ectmphoresis，2-DE）图谱。从多个差异蛋白质点中选择出2个在NB4-R1细胞株中表达明显上调的蛋白质点进行MALDI-TOF-MS生物质谱分析，并用Mascot软件在SwissProt和NCBInr数据库中进行同源比较和分析鉴定。结果确定表达明显上调的两个蛋白质分别为原肌球蛋白（tmpomyosin，TM）和丙酮酸激酶（Pymvate kinase，PK）。结论本研究表明维甲酸耐药细胞中原肌球蛋白和丙酮酸激酶表达上调，提示它们在肿瘤耐药中有重要作用。

The up- regulation of tropomyosin and pyruvate kinase in retinoicacid resistent cells of APL

Objective To sift associated proteins of drug fast in human's acute promyelocytic leukemia(APL) retinoicacid sensitive cell strain(NB4 cell strain) and retinoicacid resistent cell strain(NB4-R1 cell strain) by means of proteomic methods,so as to provide experimental foundation and theoretical basis of retinoicacid's drug fast reversion.Methods The total proteins were compared in NB4 and NB4-R1 cell strains by two-dimensional polyacrylamide gel electrophoresis detection and two-dimensional polyacrylamide gel electrophoresis atlas was established.2 significantly upregulated protein spots in NB4-R1 cell strain selected from several differentia protein spots were analyzed with biologic mass spectrometry technique MALDI-TOF-MS,and homologously compared and identified in SwissProt and NCBInr databases with Mascot software.Results The two proteins significantly upregulated were ascertained to be Tm(Tropomyosin) and PK(Pyruvate kinase).Conclusion The research shows that Tm and PK are significantly upregulated in retinoicacid resistent cells,which infers that Tm and PK play an important role in drug fast of neoplasm.