化疗患者口腔白假丝酵母菌的分布及基因分组研究

目的研究以白假丝酵母菌（S.albicans）为代表的假丝酵母菌在化疗肿瘤患者口腔中的分布情况，并对检出的白假丝酵母菌进行基因分组。方法从390例接受化疗1年以上的肿瘤患者口腔中取样，采用颊黏膜拭子法，CHROMagar CandidaTM鉴定培养基初步鉴定，根据白假丝酵母菌25S rDNA基因多态性设计引物，根据PCR产物多态性进行基因分组。结果肿瘤患者口腔假丝酵母菌检出率为53.85％（210/390），白假丝酵母菌的检出率为48.21％（188/390），其余为光滑假丝酵母菌5.64%（22/390）；白假丝酵母菌基因分组A、B、C组均有检出，其中以B组为主，59.57%（112/188）。结论肿瘤患者口腔的假丝酵母菌以白假丝酵母菌为主，与健康人群的口腔白假丝酵母菌A组占有绝对多数的情况不同，B组白假丝酵母菌在化疗患者口腔中占主导地位。

Distribution and genotypic subgroup of oral Saccharomyces albicans isolated from cancer patients receiving chemotherapy

Objective The objective of this study is to study the distribution and molecular characteristics of the oral Saccharomyces albicans（S.albicans） in the cancer patients receiving chemotherapy. Methods 390 cancer patients receiving chemotherapy were sampled by oral mucosal swab. The Candida species were identified by CHROMagar CandidaTM differential medium. All the S.albicans were genotypic grouped by PCR using primers reported to span a transposable intron region in the 25S rDNA gene. Results The frequency of oral Candida carriage of the cancer patients receiving chemotherapy was 53.85％（210/390）. Most of them were Saccharomyces albicans, the frequency was 48.21%（188/390）. The frequency of oral Candida glabrata carriage was 5.64%（22/390）. Genotypic subgroup A, B, C of Saccharomyces albicans were determined, and genotypic group B was the predominant group 59.57%（112/188）. Conclusion Saccharomyces albicans, especially genotypic subgroup B, rather than subgroup A, is the prevalence subpopulation in the oral Candida obtained from cancer patients receiving chemotherapy.