| 磷酸酰肌醇-3激酶抑制剂对小鼠气道炎症影响的实验研究 |
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| 引用本文： | 喻宁芬,喻宁芳,邓礼,于力,翁志媛,陈英,肖雪,张又祥,李书华.磷酸酰肌醇-3激酶抑制剂对小鼠气道炎症影响的实验研究[J].现代医院,2008,8(4):7-9. |
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| 作者姓名： | 喻宁芬 喻宁芳 邓礼 于力 翁志媛 陈英 肖雪 张又祥 李书华 |
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| 作者单位： | 1. 广州市第一人民医院,广东广州,510180
2. 广州医学院病理教研室,广东广州,510182 |
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| 基金项目： | 广东省科技厅科技计划
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广东省广州市医药卫生科技基金 |
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| 摘 要： | 目的观察磷酸酰肌醇-3激酶(PI3K)抑制剂LY294002对卵白蛋白(OVA)诱导哮喘小鼠气道炎症的影响。方法Balb/c小鼠30只,随机分为三组,每组10只:PI3K抑制剂治疗组(LY组),OVA组和正常对照组(NC组)。通过OVA多次腹腔注射致敏和反复雾化激发,建立哮喘小鼠模型。末次抗原激发后48h收集支气管肺泡灌洗液(BALF)和骨髓标本,计数细胞总数和嗜酸性粒细胞(Eos),并行肺组织学检查。结果与OVA组比较,LY组BALF中细胞总数及Eos绝对数明显减少(p<0.05);肺组织中细支气管、血管周围Eos浸润得到控制,气道粘液分泌减少。结论PI3K抑制剂(LY294002)对卵白蛋白(OVA)致敏的哮喘小鼠气道炎症具有抑制作用。
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| 关 键 词： | 磷酸酰肌醇-3激酶 LY294002 哮喘 气道炎症 |
PHOSPHATIDYLINOSITOL 3-KINASE INHIBITOR REDUCES RESPIRATORY TRACT INFLAMMATION IN A MURINE MODEL OF ASTHMA |
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| Affiliation: | YU Ningfen, YU Ningfang, DENG Li, et al(Guangzhou First Municipal People＇ s Hospital, Guangzhou City, Guangdong Province 510180 PRC) |
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| Abstract: | Objective To investigate the effect of phosphoinostitide 3- kinase inhibitor on bronchial inflammation in a murine model of asthma. Methods 30 BALB/c mice were randomly divided into 3 groups(10 each group): PI3K inhibitor (LY294002)-treated group, ovalbumin (OVA) group and normal control group. BALB/c mice were sensitized and challenged with OVA. Cells of bronchoalveolar larvage fluid( BALF) and bone marrow were counted and classified 2 days post last OVA sensitization. Lung tissues were sectioned to hematoxylin-eosin and periodic acid schiff staining. Results LY294002 treatment showed reduced OVA-induced airway infiltration of inflammatory cells and eosinophils in BALF; little inflammatory infiltration and eosinophils numbers around airway, blood vessels or in lung histological sections. Conclusion These findings provide the potential role of PI3K inhibitor in treatment of OVA-induced asthma by attenuating airway inflammation. |
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| Keywords: | Phosphoinostitide 3-kinase(PI3K) LY294002 Asthma Airway inflammation |
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