| 羊栖菜多糖诱导肿瘤细胞凋亡的研究 |
| |
| 引用本文： | 季宇彬,高世勇,张秀娟. 羊栖菜多糖诱导肿瘤细胞凋亡的研究[J]. 中国中药杂志, 2004, 29(3): 245-247 |
| |
| 作者姓名： | 季宇彬 高世勇 张秀娟 |
| |
| 作者单位： | 1. 哈尔滨商业大学,药物研究所,博士后科研工作站,黑龙江,哈尔滨,150076
2. 哈尔滨商业大学,药物研究所,博士后科研工作站,黑龙江,哈尔滨,150076;中国药科大学,生理教研室,江苏,南京,210009 |
| |
| 基金项目： | 国家自然科学基金，教育部科学技术基金，黑龙江省自然科学基金，黑龙江省高校骨干教师创新能力资助计划 |
| |
| 摘 要： | 目的 :揭示羊栖菜多糖 (SFPS)的抗肿瘤作用机制。方法 :采用流式细胞仪观察羊栖菜多糖对肿瘤细胞周期及细胞凋亡的影响。采用Fluo-3/AM探针标记 ,激光共聚焦技术观测细胞内 [Ca2+ ]i。结果 :羊栖菜多可阻滞SGC-7901人胃癌细胞由G0 /G1期进入S期 ,升高细胞凋亡指数 (APO% )。可使SGC-790 1细胞内 [Ca2+ ]i先升高然后下降 ,给CaCl2 后 ,[Ca2+ ]i又升高。结论 :羊栖菜多糖通过升高肿瘤细胞内 [Ca2+ ]i启动肿瘤细胞凋亡机制而达到抗肿瘤的作用 ,[Ca2+ ]i升高时Ca2+ 来源于细胞内钙库释放。
|
| 关 键 词： | 羊栖菜多糖 SGC-7901 细胞凋亡 [Ca~(2+)]i 抗肿瘤 |
| 文章编号： | 1001-5302(2004)03-0245-03 |
| 收稿时间： | 2003-09-18 |
Influence of sargassum fusiforme polysaccharide on apoptosis of tumor cells |
| |
| JI Yu-bin ;GAO Shi-yong ;ZHANG Xiu-juan. Influence of sargassum fusiforme polysaccharide on apoptosis of tumor cells[J]. China Journal of Chinese Materia Medica, 2004, 29(3): 245-247 |
| |
| Authors: | JI Yu-bin GAO Shi-yong ZHANG Xiu-juan |
| |
| Affiliation: | Postdoctoral Programme, the Institute of Material Medica of Harbin University of Commerce, Harbin 150076, China. jyb@hrbcu.edu.cn |
| |
| Abstract: | Objective: To study mechanism of antitumor a ctivity of Sargassum Fusiforme Polysaccharide(SFPS). Method: The effect on cell cycle and apoptosis was studied with flow cytometry(FCM). Intracellular calcium concentration [Ca2+ ]i was marked with Fluo-3/AM and measured with laser scanner confocal microscope(LSCM). Result: SFPS inhibited G 0/G1 stage SGC-7901 from entering to S stage an d increased APO%. The [Ca 2+ ]i showed a transient rise and return to th e original level. The concentration could be raised again by administering CaCl2. Conclusion:The antitumor effect of SFPS seems to be accomplished through the apoptosis associated with the increase in intracellular calcium concentration. Intracellular stores release the calcium during its acti on. |
| |
| Keywords: | SFPS SGC-7901 apoptosis [Ca2+ ]i anti-tumor activities |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
