HPLC－紫外法测定人血浆中芬太尼浓度

目的建立高效液相色谱法－紫外检测器测定人血浆中芬太尼浓度的方法。方法本实验采用外标法，以Shim－PackCLC－ODS（60mm×150mm，5μm）为固定相，含0015mol/LNaH2PO4的乙腈－水溶液（30∶70，v/v）为流动相，流速15ml/min，紫外检测波长195nm。结果标准曲线在20～100ng/ml范围内线性关系良好（r=0999），最低检测浓度为1ng/ml，方法回收率为（9170±470）％，提取回收率为（9738±369）％，日内变异RSD（650±279）％，日间变异RSD（670±304）％。结论本方法简便，准确，检测浓度低，能够满足血浆中低浓度芬太尼的测定及临床药代动力学研究的要求。

Determination of Concentration of Fentanyl in Human Plasma by HPLC With UV Detection

OBJECTIVE： To determine the concentration of fentanyl in human plasma by HPLC with ultraviolet detection METHODS： External standard method was used The eluation of fentanyl was carried out by shim－ Pack CLC－ ODS（ 6 0mm× 150mm， 5μ m） column with 0 015mol/L NaH2PO4 acetonitril－ H2O（ 30∶ 70， v/v） as the mobile phase， and detection wavelength was 195nm RESULTS： Calibration curve for fentanyl was linear in the range of 2 0～ 100ng/ml（ r=0 999）  The detection limit was 1 0ng/ml The within－ day RSD was （ 6 50± 2 79） ％ ， the between－ day RSD Was （ 6 70± 3 04） ％  The method recovery was （ 91 70± 4 70） ％  The extraction recovery was （ 97 38± 3 69） ％  CONCLUSION： This method was quick and accurate for the determination of fentanyl in human plasma and useful for pharmakinetic study of fentanyl