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| 盾叶薯蓣离体培养体系的建立及薯蓣皂苷元含量分析 | |
| 引用本文： | 谢彩侠,崔永霞,高山林,秦惠贞. 盾叶薯蓣离体培养体系的建立及薯蓣皂苷元含量分析[J]. 中国中医药现代远程教育, 2009, 7(2) |
| 作者姓名： | 谢彩侠 崔永霞 高山林 秦惠贞 |
| 作者单位： | 1. 河南中医学院药学院,河南,郑州,450008 2. 中国药科大学中药学院,江苏,南京,210038 3. 中国科学院植物研究所,江苏,南京,210041 |
| 摘 要： | 以盾叶薯蓣叶片为外植体,研究不同浓度激素组合对盾叶薯蓣愈伤组织、不定芽及再生植株的影响,并对不同材料诱导获得的再生植株中薯蓣皂苷元含量进行了分析。结果表明,诱导愈伤组织的最适培养基为MS+BA2.0mg·L-1+2,4D0.1mg·L-1;诱导不定芽及芽生长的培养基的最适培养基为MS+BA0.1mg·L-1(0.05mg·L-1)+病毒唑5mg·L-1;诱导试管苗生根的最适培养基为MS/2+IAA0.2mg·L-1+NAA0.2mg·L-1。相同外植体诱导的再生植株中薯蓣皂苷元含量差异不明显;不同盾叶薯蓣诱导的再生植株中薯蓣皂苷元含量差异明显。 |
| 关 键 词： | 盾叶薯蓣 愈伤组织 不定芽 薯蓣皂苷元 |
Establishment of Culture System of Dioscorea zingibiernsis C.H.Wright and Content of the Diosgenin in Its Regenerated Plantlets |
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| Xie Cai-xia,Cui Yong-xia,Gao Shan-lin,Qin Hui-zhen. Establishment of Culture System of Dioscorea zingibiernsis C.H.Wright and Content of the Diosgenin in Its Regenerated Plantlets[J]. Chinese Medicine Modern Distance Education of China, 2009, 7(2) | |
| Authors: | Xie Cai-xia Cui Yong-xia Gao Shan-lin Qin Hui-zhen |
| Affiliation: | 1.He’nan College of Traditional Chinese Medicine; Zhengzhou 450008; China 2.China Pharmaceutical University; Nanjing; 210038; China 3.Institute of Botany; Jiang su Province and the Chinese Academy of Sciences; 210014; China); |
| Abstract: | The leaves of Dioscorea zingibiernsis C. H.Wright were used as the explants to study the callus induction 、shoot induction and plant regeneration on the MS media containing different hormone combinations. On the basis of it, content of the diosgenin in regenerated plantlets from different sources were analyzed .The results indicated that the callus induction was better on MS medium with 2.0 mg/L 6-BA and 1.0 mg/L2,4D; the shoot induction and growth was better on MS medium added with 0.1(0.05) mg/L 6-BA and ... |
| Keywords: | Dioscorea zingibiernsis C. H.Wright Callus tissue Adventitious bud Diosgenin |
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