miRNA-141在弥漫大B细胞淋巴瘤中的表达及其与预后的关系

目的 探讨miRNA 141在弥漫大B细胞淋巴瘤（diffuse large B cell lymphoma, DLBCL）中的表达及其与预后的关系。方法 选取锦州医科大学附属第一医院2017年1月-2019年12月收治的40例DLBCL患者初始治疗前的石蜡包埋组织标本。应用荧光实时定量 PCR法检测其中miRNA 141的表达水平。同时按照免疫分型、国际预后指数（IPI评分）等分组,探讨miRNA 141的相对表达量与免疫分型等临床特征之间的关系。另收集性别、年龄相匹配的20例淋巴结反应性增生（reactive hyperplasia of lymph node, RHL）患者的石蜡组织标本作为对照组。结果 miRNA 141在DLBCL患者中的表达水平低于RHL组（P＜0.05）。DLBCL中miRNA 141高表达组的2年总体生存率（92.2%）高于低表达组（26.2%）（P=0.001）。多因素 COX 模型分析显示:40例 DLBCL中, miRNA 141低表达（P=0.009）、国际预后指数IPI≥3分（P=0.012）分别为各自独立的预后不良的因素。结论 miRNA 141在弥漫大B细胞淋巴瘤患者中表达下降, miRNA 141高表达组总体生存率明显高于低表达组,提示miRNA 141可能是辅助诊断及评估预后的另一个标志物。

Expression of mirna 141 in diffuse large B cell lymphoma and its relationship with prognosis

Objective To investigate the expression of miRNA 141 in diffuse large B cell lymphoma (DLBCL) and its relationship with prognosis. Methods Paraffin embedded tissue specimens of 40 patients with DLBCL admitted to the First Affiliated Hospital of Jinzhou Medical University from January 2017 to December 2019 before initial treatment were selected. The expression level of miRNA 141 was detected by fluorescence real time quantitative PCR. At the same time, the relationship between the relative expression level of miRNA 141 and the clinical characteristics was investigated according to the groups of immune typing and international prognostic index (IPI score).In addition, we selected paraffin tissue samples from 20 patients with reactive hyperplasia of lymph node(RHL) matched by gender and age as the control group. Results The expression level of miRNA 141 in DLBCL patients was lower than that in RHL group(P<0.05). The 2 year overall survival rate (92.2%) of the group with high expression of miRNA 141 in DLBCL was higher than 26.2% of the group with low expression(P=0.001). Multivariate COX model analysis showed that the low expression group of miRNA 141 (P=0.009) and the international prognostic index IPI≥3 (P=0.012) were the independent prognostic factors among 40 cases of DLBCL. Conclusion The expression of miRNA 141 is decreased in patients with DLBCL, and the overall survival rate of the group with high expression of miRNA 141 was significantly higher than that of the group with low expression, suggesting that miRNA 141 may be another marker for assisting diagnosis and evaluating prognosis.