CD90在肝内胆管细胞癌中的表达及其与患者预后不良的关系

目的 探讨分析CD90在人肝内胆管细胞癌中的表达及与淋巴结转移和预后不良的关系。方法 收集2013年3月-2016年3月承德医学院附属医院手术切除的肝内胆管细胞癌组织标本49例及其癌旁正常组织49例,另选取40例正常肝组织作为对照。采用免疫组化法检测所有标本CD90表达情况,并分析肝内胆管细胞癌患者CD90表达与临床指标、生存预后的关系。计量资料2组间比较采用t检验;计数资料2组间比较采用χ2检验,3组间比较及进一步两两比较均采用Kruskal-Wallis H检验。采用Kaplan-Meier曲线分析术后复发及生存情况,并应用log-rank检验进行比较。结果 肝内胆管细胞癌组织CD90中强度阳性表达率为65.31%,癌旁正常组织CD90中强度阳性表达率为30.61%,正常肝组织CD90中强度阳性表达率为0,肝内胆管细胞癌组织中CD90蛋白中强度阳性表达率显著高于癌旁正常组织与正常肝组织(P值均<0.05)。CD90蛋白表达与肝内胆管细胞癌患者TNM分期及肝门淋巴结转移有关(χ^2值分别为12.837、17.824,P值均<0.001)。CD90蛋白阴性/弱阳性表达患者术后无复发生存率及总体生存率均显著优于中/强阳性表达患者(χ^2值分别为3.845、4.152,P值分别为0.025、0.021)。结论 CD90在人肝内胆管细胞癌组织内高表达,且与患者肝门淋巴结转移及不良预后有关,在肿瘤发生发展及转移过程中发挥着重要作用。

Expression of CD90 in intrahepatic cholangiocellular carcinoma and its association with poor prognosis

Objective To investigate the expression of CD90 in human intrahepatic cholangiocellular carcinoma and its association with lymph node metastasis and poor prognosis. Methods A total of 49 samples of intrahepatic cholangiocellular carcinoma tissue and 49 samples of adjacent tissue were collected after surgical resection in The Affiliated Hospital of Chengde Medical College from March 2013 to March 2016, and 40 normal liver tissue samples were selected as control. Immunohistochemistry was used to measure the expression of CD90 in each sample, and its association with clinical indices, survival, and prognosis of patients with intrahepatic cholangiocellular carcinoma was analyzed. The t-test was used for comparison of continuous data between two groups;the chi-square test was used for comparison of categorical data between two groups, the Kruskal-Wallis H test was used for comparison between three groups and further comparison between two groups. The Kaplan-Meier curve was used to analyze postoperative recurrence and survival, and the log-rank test was used for comparison. Results The moderately positive expression rate of CD90 was 65.31% in intrahepatic cholangiocellular carcinoma tissue, 30.61% in adjacent normal tissue, and 0% in normal liver tissue, suggesting that the intrahepatic cholangiocellular carcinoma tissue had a significantly higher moderately positive expression rate of CD90 than adjacent normal tissue and normal liver tissue (P<0.05). The expression of CD90 was associated with TNM stage and hilar lymph node metastasis in the patients with intrahepatic cholangiocellular carcinoma (χ^2=12.837 and 17.824, both P<0.001). The patients with negative/weakly positive expression of CD90 had significantly higher relapse-free survival rate and overall survival rate after surgery than those with moderately/strongly positive expression (χ^2=3.845 and 4.152, P=0.025 and 0.021). Conclusion CD90 is highly expressed in human intrahepatic cholangiocellular carcinoma tissue, which is associated with hilar lymph node metastasis and poor prognosis and plays an important role in tumor development, progression, and metastasis.