肝细胞癌患者超声造影特征与其肿瘤标志物表达的临床研究

目的分析肝细胞癌(HCC)患者超声造影特征与其肿瘤标志物表达情况,探讨其临床应用价值。方法选取术前行超声造影检查,并经手术病理证实的214例HCC患者,使用免疫组化染色观察白细胞介素8(IL-8)和甲胎蛋白(AFP)的表达情况,分析超声造影参数(始增时间、达峰时间、峰值加速时间、始增速度及增强速率)与肿瘤标志物之间的关系,以及IL-8、AFP表达情况与HCC的关系。结果 HCC病灶的始增时间、达峰时间、峰值加速时间均小于病灶周围背景肝组织,病灶始增速度和增强速率均大于病灶周围背景肝组织,差异均有统计学意义(均P<0.05)。IL-8表达阳性率74.77%(160/214),AFP表达阳性率69.62%(149/214),两者表达呈正相关(r=0.252,P=0.003)。IL-8、AFP阴性HCC患者的始增时间、达峰时间、峰值加速时间均大于IL-8、AFP阳性患者,差异均有统计学意义(均P<0.05);IL-8、AFP阳性HCC患者的始增速度、增强速率均大于IL-8、AFP阴性患者,差异均有统计学意义(均P<0.05)。结论 HCC超声造影特征可为HCC肝切除患者的生物学行为评估、临床预处理及预后评估提供依据,具有较好的临床应用价值。

Clinical study of contrast-enhanced ultrasound characteristics and tumor markers expression in patients with hepatocellular carcinoma

ObjectiveTo investigate the contrast-enhanced ultrasound characteristics and tumor markers expression in patients with hepatocellular carcinoma(HCC),and to explore its clincal application value.MethodsA total of 214 patients with HCC confirmed by surgery or puncture pathology who had received contrast-enhanced ultrasonography before surgery were collected.The expression of interleukin-8(IL-8)and alpha fetoprotein(AFP)was observed by immunohistochemical staining.The relationship between the parameters of contrast-enhanced ultrasound(onset time,peaking time,peak acceleration time,initiation rate and enhancement rate)and tumor markers was analyzed,as well as the relationship between the expression of IL-8/AFP and HCC.ResultsThe onset time,peaking time and peak acceleration time of the HCC lesions were less than those of background liver tissue around the lesions,and the initiation rate and enhancement rate of HCC were higher than those of background liver tissue around the lesions(all P<0.05).The positive rate of IL-8 expression was 74.77%(160/214),and the positive rate of AFP expression was 69.62%(149/214).There was a significant positive correlation between the two expressions(r=0.252,P=0.003).The onset time,peaking time and peak acceleration time of IL-8/AFP negative HCC were greater than those of IL-8/AFP positive HCC(all P<0.05).Initiation rate and enhancement rate of IL-8/AFP positive HCC were greater than those of IL-8/AFP negative HCC(all P<0.05).ConclusionThe contrast-enhanced ultrasound characteristics of HCC can provide the basis for biological behavior evaluation,clinical pretreatment and prognosis in patients with HCC hepatectomy,which has better clinical application value.