凋亡调节蛋白XIAP与Omi/HtrA2在弥漫大B细胞淋巴瘤中的表达及其与患者预后的关系

目的 探讨XIAP与Omi/HtrA2在弥漫大B细胞淋巴瘤（DLBCL）组织中的表达及与其预后的相关性。方法 收集59例DLBCL患者和30例淋巴结反应增生(RHL)患者的石蜡病理切片，应用免疫组化法对XIAP和Omi/HtrA2进行染色，分析两种蛋白的表达与DLBCL的临床参数和预后的关系。结果 XIAP与Omi/HtrA2在DLBCL中均呈高表达，在RHL中呈低表达甚至未见表达。XIAP在DLBCL的平均阳性率为61.02%，在RHL平均阳性率为36.67%（P＜0.05）。Omi/HtrA2在DLBCL中平均阳性率为52.54%，RHL平均阳性率为30.00%（P＜0.05）。二者的阳性表达与DLBCL的临床分期、病理类型、乳酸脱氢酶（LDH）水平、淋巴瘤国际预后指数（IPI）评分相关（P＜0.05），与性别、年龄、症状分组、结外受累无关（P＞0.05）。XIAP和Omi/HtrA2表达呈负相关（P＜0.05）。Kaplan meier生存分析显示，XIAP的表达与DLBCL患者的预后相关（P＜0.05）。Omi/HtrA2与DLBCL预后不相关（P＞0.05）。结论 XIAP与Omi/HtrA2在DLBCL组织中均呈高表达，二者呈负相关，上调Omi/HtrA2抑制XIAP表达可为DLBCL靶向治疗提供依据，XIAP是DLBCL预后相关性因素，有望成为判断DLBCL预后的指标。

Expression of apoptosis regulating proteins XIAP and Omi/HtrA2 in diffuse large B cell lymphoma and relationship with prognosis

Objective To investigate expression and prognosis of XIAP and Omi/HtrA2 in diffuse large B cell lymphoma (DLBCL) and the relationship with prognosis.Methods Paraffin sections were collected from 59 patients with DLBCL and 30 patients with lymph node hyperplasia (RHL).The XIAP and Omi/HtrA2 proteins in both groups were stained by immunoh is to chemical method, the relationship of their expressions with clinical parameters and prognosis of DLBCL were analyzed.Results XIAP and Omi/HtrA2 were highly expressed in DLBCL, but lower expressed or even not expressed in RHL. The average positive rate of XIAP in DLBCL was 61.02%, and that of RHL was 36.67%(P＜0.05). The average positive rate of Omi/HtrA2 in DLBCL was 52.54%, and that of RHL was 30.00%(P＜0.05). The positive expressions of XIAP and Omi/HtrA2 were associated with clinical stage, pathological type, LDH level, and IPI score of DLBCL(P＜0.05), but were not associated with gender, age, extranodal involvement, and symptom grouping(P＞0.05).The expressions of XIAP and Omi/HtrA2 were negatively correlated(P<0.05). Kaplan meier survival analysis showed that the expression of XIAP was correlated with the prognosis of patients with DLBCL(P＜0.05),but the expression of Omi/HtrA2 was not correlated with the prognosis of DLBCL patients(P＞0.05).Conclusion XIAP and Omi/HtrA2 are both highly expressed in DLBCL, and there are negatively correlated. The upregulation of Omi/HtrA2 and the suppression of XIAP expression can provide a basis for DLBCL targeted therapy. XIAP, a prognostic factor related to DLBCL, will expect to be an indicator of DLBCL prognosis.