胆宁片治疗豚鼠胆色素结石的实验研究

目的]研究胆宁片治疗豚鼠胆色素结石的作用及机制。方法]构建豚鼠胆色素结石模型,给予胆宁片小、中、大剂量治疗,治疗后观察豚鼠成石率,并用ELISA法测定血清中IL-15、IL-1β及胆汁中IL-15含量。结果]胆宁片中、大剂量治疗后的豚鼠成石率较模型组下降,差异有统计学意义（P〈0.01）;与模型组比较,胆宁片各剂量组血清中IL-15、IL-1β及胆汁中IL-15含量减少,差异有统计学意义（P〈0.01）,且胆宁片各剂量组间比较差异也有统计学意义（P〈0.01）。结论]胆宁片能有效治疗豚鼠胆色素结石,其机制与减少IL-15、IL-1β含量有关。

The experimental study of Danning Tablet on guinea pigs with bile pigment calculusThe experimental study of Danning Tablet on guinea pigs with bile pigment calculus

Objective]To explore the therapeutic effect and the mechanism of Danning Tablet on guinea pigs with bile pigment calculus. Methods]We established the model of guinea pigs with bile pigment calculus and used Danning Tablets of low, medium and high doses to cure them. After treatment, the rate of gallstone and the content of IL-15 and IL-1β in the serum as well as the content of IL-15 in the bill were measured with enzyme-linked immunosorbent assay （ELISA） method. Results]Compared with the model group,the rate of gallstone in medium and high dose Danning Tablet groups was lower than that of model group（P〈0.01） Compared with th6 model group,the content of IL-15 in the serum and bile as well as the content of IL-I~ in the serum were significantly decreased, and there was significant difference（-P〈0.01） There was also significant difference among three-dose groups（P〈.01）. Conclusion]Danning Tablet has the effects of treating guinea pigs with bile pigment calculus. The mechanism is related to the decrease of the content of IL-15 and IL-1β.