| 番茄红素对大气可吸入颗粒物致人肺成纤维细胞DNA损伤的保护作用 |
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| 引用本文： | 姜薇,赵晓红,米生权,李爽,王健,贺旭.番茄红素对大气可吸入颗粒物致人肺成纤维细胞DNA损伤的保护作用[J].劳动医学,2008,25(6). |
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| 作者姓名： | 姜薇 赵晓红 米生权 李爽 王健 贺旭 |
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| 作者单位： | 北京联合大学应用文理学院生物活性物质与功能食品北京市重点实验室; |
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| 基金项目： | 北京市自然科学摹金
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| 摘 要： | 目的]探讨大气粗颗粒物(PM10)、细颗粒物(PM2.5)对人肺成纤维细胞(HLF)增殖作用的影响和DNA链的损伤作用以及番茄红素的保护作用.方法]采用细胞增殖试验(MTT)测定PM10、PM25以及番茄红素对HLF增殖作用.单细胞凝胶电泳法(SCGE)检测PM10、PM25对HLF DNA的损伤程度以及番茄红素对DNA损伤的保护作用.结果]PM10、PM25均能抑制HLF的增殖,当颗粒物质量浓度为50μg/mL时,HLF的细胞存活率下降约10%,随着染毒剂量的增大细胞存活率进一步下降,具有明显的剂量一效应关系(P<0.01).同样质量浓度下比较,PM25对细胞增殖的抑制作用大干PM10;PM10和PM25均具有明显的DNA损伤作用,当PMs质量浓度仅为50μg/mL时,即能够引起细胞DNA损伤(P<0.01).且具有明显的剂量-反应关系(P<0.01),但PM25的损伤程度小于PM10;番茄红素可以有效地阻止由PM10、PM25导致的DNA损伤,浓度为25μmol/L的番茄红素即可明显减轻PMs对HLF造成的DNA损伤.结论]PMs能抑制HLF的增殖、诱导HLF的DNA链断裂,番茄红素可以有效地减小PMs对HLF DNA造成的损伤.
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| 关 键 词： | 大气可吸入颗粒物 DNA损伤 单细胞凝胶电泳 番茄红素 |
Effect of DNA Damage Exposed to Ambient Particle Matters and Protecting Effect of Lycopene in HLF Cells |
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| JIANG Wei,ZHAO Xiao-hong,MI Sheng-quan,LI Shuang,WANG Jian,HE Xu.Effect of DNA Damage Exposed to Ambient Particle Matters and Protecting Effect of Lycopene in HLF Cells[J].Journal of Labour Medicine,2008,25(6). |
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| Authors: | JIANG Wei ZHAO Xiao-hong MI Sheng-quan LI Shuang WANG Jian HE Xu |
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| Institution: | JIANG Wei,ZHAO Xiao-hong,MI Sheng-quan,LI Shuang,WANG Jian,HE Xu(Beijing Key Lab of Bioactive Substances , Functional Foods,College of Arts , Science,Beijing Union University,Beijing 100083,China) |
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| Abstract: | Objective]To investigate the effect on cell proliferation and DNA damage of human lung fibroblasts (HLF)exposed to ambient coarse(PM10)and fine(PM2.5)particle matters and to study the protective effect of lycopene on DNA damage exposed to PM10 and PM2.5.Methods] The effects of PM10,PM2.5 and lycopene on HLF proliferation were measured by MTT Assay. The degree of DNA damage exposed to PM10,PM2.5 and the protective effects of lycopene on DNA injury by PM10 and PM2.5 were determined by the single cell gel el... |
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| Keywords: | ambient respirable particulate DNA damage single cell gel electrophoresis(SCGE) lycopene |
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