子宫内膜癌腺体及间质细胞中基质细胞衍生因子1及其受体CXCR4的表达

目的:检测基质细胞衍生因子1(SDF-1)及其受体CXCR4 mRNA和蛋白在子宫内膜癌细胞系及组织中的表达.方法:应用免疫组织化学和RT-PCR技术,检测子宫内膜癌细胞系Ishikawa和HEC-1A细胞,44例子宫内膜癌组织,26例非典型增生子宫内膜、单纯增生子宫内膜和正常子宫内膜组织中SDF-1和CXCR4 mRNA和蛋白表达水平.结果:HEC-1A和Ishikawa细胞均表达CXCR4 mRNA;Ishikawa细胞中SDF-1 mRNA弱表达,而HEC-1A细胞中SDF-1 mRNA未见表达.CXCR4和SDF-1在正常和良性病变的子宫内膜组织腺体细胞中的阳性表达率为100%,在子宫内膜癌组织的腺体细胞中的阳性表达率分别为90.9%和93.2%.各种子宫内膜组织中间质细胞CXCR4和SDF-1的表达均显著弱于腺体细胞.在子宫内膜癌腺体细胞和间质细胞中CXCR4和SDF-1的表达均显著弱于正常及良性病变者.在低分化的子宫内膜癌腺体细胞中CXCR4和SDF-1的表达弱于高分化者.结论:CXCR4和SDF-1在子宫内膜癌细胞系、子宫内膜组织及内膜癌组织中的表达存在差异,在内膜癌组织中其表达均显著弱于正常及良性病变者.

Expressions of stromal cell-derived factor-1 and CXCR4 in endometrial carcinoma tissues and cell lines

Objective: To investigate the expressions of SDF-1 and CXCR4 in human endometrial carcinoma tissues. Methods: SDF-1 and CXCR4 protein expression levels were detected by immunohistochemical staining in 26 cases of atypic hyperplasia endometrium,simple hyperplasia endometrium and normal endometrium and 44 cases of endometrial carcinoma tissues. Results: Both SDF-1 mRNA and CXCR4 mRNA were expressed in Ishikawa, while no expression of SDF-1 mRNA was detected in HEC-1A.The rates of CXCR4 and SDF-1 protein expressions were 100% in glandular cells of human normal endometrial tissues, while 90.9% and 93.2% in glandular cells of endometrial carcinoma tissues.These immunoreactivities of SDF-1 and CXCR4 were significantly low in endometrial carcinoma tissues as compared with those in normal endometrium;and the intensities of immunohistochemical staining significantly decreasesd in immunoreactivity of SDF-1 and CXCR4 in low grade as compared with that in hight grade. Conclusion: SDF-1 and CXCR4 were expressed differently in endometrial cell lines, human normal endometrial tissues,and endometrial carcinoma tissues. As compared with normal endometrium ,these were significantly low levels expression of SDF-1 and CXCR4 in endometrial carcinoma tissues.