p53基因与川崎病患者淋巴细胞凋亡关系探讨 |
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引用本文: | 易岂建,杨锡强,李成荣,张远维,王莉佳.p53基因与川崎病患者淋巴细胞凋亡关系探讨[J].重庆医科大学学报,2001,26(1):51-52,55. |
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作者姓名: | 易岂建 杨锡强 李成荣 张远维 王莉佳 |
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作者单位: | 1. 重庆医科大学儿科学院 2. 广东省深圳市儿童医院 |
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摘 要: | 目的:进一步探讨川崎病(KD)急性期患者外周血淋巴细胞凋亡延迟的机理。方法:采用斑点杂交(Dot-blot)检测淋巴细胞p53基因mRNA表达水平;流式细胞仪(FCM)检测p53蛋白质表达阳性细胞百分率。结果:KD患者外周血淋巴细胞p53基因mRNA和p53蛋白质表达水平降低,与正常儿童比较差异显著(P<0.005);当给予静脉注射免疫球蛋白(IVIG)治疗后或加入抗IL-6单抗培养时,外周血淋巴细胞p53基因mRNA和P53蛋白质表达水平提高。结论:KD急性期患者外周血淋巴细胞p53基因表达水平降低,其原因可能与本病患者异常升高的IL-6有关。p53基因具有促进细胞凋亡的作用,KD患者外周血淋巴细胞凋亡延迟可能与高浓度IL-6抑制p53基因的表达有关。
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关 键 词: | 川崎病 细胞凋亡 p53基因 白细胞介素-6 |
文章编号: | 0253-3626(2001)01-0051-02 |
The expression of p53 gene in peripheral blood lymphocyte of acute Kawasaki disease |
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Abstract: | Objective: To further explore the mechanism of inhibited apoptosis of peripheral blood mononuclear cell (PBMC) in acute Kawasaki disease(KD). Methods: The expression level of p53 gene mRNA was determined by dot-blot; p53 protein positive cell percentage was detected by flow cytometry (FCM). Results:The expression of p53 gene mRNA and p53 protein in acute KD patients were decreased(P<0.001), but increased after treating with intravenous immunoglobulin(IVIG) in vivo or adding anti-IL-6 monoantibody(mAb)into PBMC culture in vitro. Conclusion. The decreased expression of p53 gene mRNA and p53 protein may be associated with the high concentration of IL-6 in KD patients, p53 gene expression could induce lymphocyte apoptosis. Thus, the expression of p53 gene inhibited by the increased IL-6 production might be related to delaying or depressing apoptosis of PBMC in KD. |
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