| 超声破坏微泡介导骨髓间充质干细胞移植的实验研究 |
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| 引用本文： | 白园园,修建成,查道刚,王月刚,刘伊丽.超声破坏微泡介导骨髓间充质干细胞移植的实验研究[J].南方医科大学学报,2009,29(2):199. |
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| 作者姓名： | 白园园 修建成 查道刚 王月刚 刘伊丽 |
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| 作者单位： | 南方医科大学南方医院心血管内科,广东,广州,510515 |
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| 摘 要： | 目的 初步探讨超声破坏微泡介导骨髓间充质干细胞(MSCs)移植的可行性.方法 21只Wister大鼠分为3组:干细胞静脉移植组(MSCs-iv)、超声+干细胞组(US+MSCs-iv)、超声+微泡+干细胞组(US+MB+MSCs-iv).各组分别取1只大鼠的骨骼肌行HE染色观察显微结构.移植后7 d取各组剩余大鼠下肢股薄肌、半膜肌行免疫组化检测,观察MSCs移植数量;另外US+MB+MSCs-iv组同时取重要脏器行免疫组化检测,评估靶向性.结果 US+MB+MSCs-iv组大鼠股薄肌、半膜肌染色可见红细胞外溢到组织间隙,同时免疫组化结果显示股薄肌、半膜肌及脾脏可见较多移植的MSCs.而其他组骨骼肌未观察到阳性细胞.结论 超声靶向破坏微泡有可能成为提高干细胞移植效率的新方法.
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| 关 键 词： | 超声 微泡 骨髓间充质干细胞 干细胞移植 靶向性 |
Targeted delivery of bone marrow mesenchymal stem cells by ultrasound-mediated microbubble destruction |
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| BAI Yuan-yuan,XIU Jian-cheng,ZHA Dao-gang,WANG Yue-gang,LIU Yi-li.Targeted delivery of bone marrow mesenchymal stem cells by ultrasound-mediated microbubble destruction[J].Journal of Southern Medical University,2009,29(2):199. |
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| Authors: | BAI Yuan-yuan XIU Jian-cheng ZHA Dao-gang WANG Yue-gang LIU Yi-li |
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| Institution: | BAI Yuan-yuan,XIU Jian-cheng,ZHA Dao-gang,WANG Yue-gang,LIU Yi-li Department of Cardiology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To investigate the feasibility of bone marrow mesenchymal stem cell (MSC) transplantation with ultrasound-targeted microbubble destruction. Methods Twenty-one Wistar rats were divided into MSCs-iv group (MSCs-iv), ultrasound+MSCs-iv group (US+MSCs-iv), ultrasound+microbubble+MSCs-iv group (US+MB+MSCs-iv) with intravenous MSC transfer, ultrasound and microbubble treatment as indicated. The skeletal muscles were obtained from the rats for microscopic examination with HE staining. The hindlimb gracil... |
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| Keywords: | ultrasound microbubble marrow mesenchymal stem cells stem cell transplantation targeting |
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