Effect of polychlorinated biphenyls on the immune responses of rhesus monkeys and mice. |
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Authors: | P T Thomas R D Hinsdill |
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Institution: | Department of Human Physiology and Pharmacology, University of Adelaide, Adelaide, South Australia 5000, Australia |
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Abstract: | The relationship between lipid peroxidation, glutathione (GSH) content, and CCl4-induced toxicity was investigated in rat hepatocytes isolated by a collagenase-perfusion technique. Two chemical initiators of lipid peroxidation, ferric ions complexed with adenosine diphosphate () and diethyl maleate, were studied for comparison. CCl4 caused a reduction of intracellular K+ and release of alanine aminotransferase (ALT) into the medium, but no evidence of lipid peroxidation, as measured by the absorbance of thiobarbituric acid (TBA)-reacting materials and lipid-extract diene conjugation. caused lipid peroxidation, but only a small loss of K+. Diethyl maleate caused a greater amount of lipid peroxidation and cell damage than did . Neither response appeared to be related to the GSH content, which was reduced by diethyl maleate, but not by , and by CCl4 only at the highest dose. The results suggest that lipid peroxidation is not a requisite step in CCl4-induced toxicity in isolated hepatocytes. |
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Keywords: | To whom correspondence should be addressed |
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