鸦胆子油乳注射液对C6胶质瘤细胞作用的实验研究

背景与目的:鸦胆子油乳注射液在临床上用于许多肿瘤的治疗,然而其确切的分子机制目前尚不完全清楚。本研究探讨鸦胆子油乳注射液对C6胶质瘤细胞增殖作用的影响。方法:体外培养大鼠C6胶质瘤细胞,用MTT比色法检测鸦胆子油乳注射液对C6胶质瘤细胞的抑制作用。用免疫组化检测鸦胆子油乳注射液对C6胶质瘤细胞caspase-3表达的影响。结果:MTT比色法显示鸦胆子油乳注射液对C6胶质瘤细胞的抑制率呈剂量依赖性。免疫组化显示随鸦胆子油乳注射液浓度增加,caspase-3表达明显增多。结论:体外鸦胆子油乳注射液能增加C6胶质瘤细胞凋亡基因caspase-3表达,抑制细胞增殖。

The Experimental Study of Effect of Brucea Javanica Oil Emulsion on C6 Glioma Cells

BACKGROUND & OBJECTIVE: Traditional Chinese medicine may be effective in the treatment of gliomas.In this study,we explored the effect of brucea javanica oil emulsion on proliferation of C6 glioma cells.METHODS: Rat C6 glioma cells were cultured in vitro.MTT colorimetric assay was used to measure the inhibitory effect of brucea javanica oil emulsion on C6 golima cells.Immunohistochemical staining was used to detect the effect of Brucea javanica oil emulsion on the caspase-3 expression of C6 glioma cells.RESULTS: MTT demonstrated that the inhibition rate of Brucea javanica oil emulsion on C6 glioma cells was dose-dependent.The immunohistochemistry showed that the caspase-3 expression of C6 glioma cells was remarkably increased with the increase of dose of brucea javanica oil emulsion.CONCLUSON: Brucea javanica oil emulsion can inhibit proliferation of C6 glioma cells by increasing the expression of caspase-3 in vitro.