家蝇幼虫抗菌蛋白对子宫内膜癌细胞生长影响

目的 探讨家蝇幼虫抗菌蛋白对子宫内膜癌细胞(JEC)的影响.方法 细胞计数法、四甲基偶氨噻唑蓝(MTT)法测定家蝇幼虫抗菌蛋白对JEC细胞生长的影响;MTT法测定家蝇幼虫抗菌蛋白对正常人牙周膜细胞生长的影响;苏木素一伊红(HE)染色法观察细胞凋亡,并计算细胞凋亡指数(AI).结果 高浓度组JEC细胞数及吸光度(A)值明显降低,细胞增殖抑制率随浓度的增加而增大;各浓度组正常人牙周膜细胞的A值和细胞增殖抑制率与对照组比较差异均无统计学意义;HE染色见细胞凋亡,各浓度组AI均明显高于对照组.结论 家蝇幼虫抗菌蛋白可能是通过诱导细胞凋亡来抑制JEC细胞生长.

Effect of antibactrial proteins from the Musca dometica larvae on the growth of JEC

Objective To explore the effect of antibactrial proteins from the Musca domestica larvae on the JEC.Methods The effect of the antibactr ial proteins from the Musca dotestlca larvae on the gowth of the JEC were observed by cytom etry,MTTassay,and its effect on the growth of periodontal membrane cell of human were observed by MTT assay.The morphology of apoptosis cells was observed with hematox ylin-eosin stainings,and the apoptosis index(AI)was measured.Results A thigh-concentr ation groups,the cell number and the optical density(OD)of JEC were significantly decreased and with the increase of the concentration,the inhibitory rate of the cell proliferation was increased.The OD and the inhibitory rate of the cell proliferation at the all-concentration groups hadnosig nificant differ ence compared with the coatrol group in the periodontal membrane cell of human.Compared with control group,the apoptosis index(AI)were significanltly increased in all concentration groups.Conclusion The antibactrial proteins from the Musca domestica.larvae may be inhibited the growth of JEC by inducing the apoptosis of cell.