| 加减八珍汤对人鼻咽癌6-10B裸鼠移植瘤生长抑制作用及瘤体组织中cyclinD1表达水平影响 |
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| 引用本文： | 陈泽南,蒿艳蓉. 加减八珍汤对人鼻咽癌6-10B裸鼠移植瘤生长抑制作用及瘤体组织中cyclinD1表达水平影响[J]. 辽宁中医药大学学报, 2019, 21(1): 40-43 |
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| 作者姓名： | 陈泽南 蒿艳蓉 |
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| 作者单位： | 广西中医药大学,广西南宁530000;广西壮族自治区人民医院,广西南宁530021;广西壮族自治区人民医院,广西南宁,530021 |
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| 基金项目： | 国家自然科学基金;广西壮族自治区研究生教育创新计划 |
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| 摘 要： | 目的:构建人鼻咽癌6-10B裸鼠移植瘤模型,给予该模型鼠加减八珍汤处理,通过HE染色、RT-RCR及Western blot,探讨该方对鼻咽癌裸鼠移植瘤生长及增殖能力的影响。方法:将10只建模成功的鼻咽癌裸鼠随机分为实验组和对照组,分别给予加减八珍汤(浓度150mg/mL)和0.9%生理盐水各0.2mL灌胃,每天两次,共14d。观察裸鼠瘤体积的变化至第21天,显微镜观察移植瘤组织病理变化特点,RT-RCR及Western blot检测移植瘤组织中cyclinD1的表达。结果:HE染色证实人鼻咽癌6-10B裸鼠移植瘤建模成功。RT-PCR和Western blot检测结果表明加减八珍汤可显著下调cyclinD1 mRNA和蛋白质的表达(P<0.05),抑制癌细胞的增殖,延缓肿瘤的生长。结论:在裸鼠体内,加减八珍汤能抑制人鼻咽癌细胞6-10B的增殖,从而达到抑制鼻咽癌组织生长的作用。
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| 关 键 词： | 鼻咽癌 加减八珍汤 6-10B细胞 CYCLIND1 增殖 |
Effects of Addition and Subtraction of Bazhen Decoction on Growth of Human Nasopharyngeal Carcinoma Cell Line 6-10B in Nude Mice and the Expression of CyclinD1 in Tumor Tissue |
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| CHEN Zenan,HAO Yanrong. Effects of Addition and Subtraction of Bazhen Decoction on Growth of Human Nasopharyngeal Carcinoma Cell Line 6-10B in Nude Mice and the Expression of CyclinD1 in Tumor Tissue[J]. Journal of Liaoning University of Traditional Chinese Medicine, 2019, 21(1): 40-43 |
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| Authors: | CHEN Zenan HAO Yanrong |
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| Affiliation: | (Guangxi University of Chinese Medicine,Nanning 530000,Guangxi,China;People's Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,Guangxi,China) |
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| Abstract: | CHEN Zenan;HAO Yanrong(Guangxi University of Chinese Medicine,Nanning 530000,Guangxi,China;People's Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,Guangxi,China) |
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| Keywords: | nasopharyngeal carcinoma addition and subtraction of Bazhen Decoction 6-10B cells cyclinD1 proliferation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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