| 桂枝挥发油干预LPS致大鼠急性肺炎模型肺Toll样受体2、4和MYD88基因表达的研究 |
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| 引用本文： | 徐世军,沈映君,金沈锐,解宇环.桂枝挥发油干预LPS致大鼠急性肺炎模型肺Toll样受体2、4和MYD88基因表达的研究[J].成都中医药大学学报,2008,31(3):32-34,37. |
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| 作者姓名： | 徐世军 沈映君 金沈锐 解宇环 |
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| 作者单位： | 成都中医药大学,四川,成都,610075;成都中医药大学,四川,成都,610075;成都中医药大学,四川,成都,610075;成都中医药大学,四川,成都,610075 |
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| 摘 要： | 目的:研究桂枝挥发油(VORC)对脂多糖(LPS)致大鼠急性肺炎模型肺Toll样受体2(111R2)、Toll样受体4(TLR4)及其下游信号转导通路髓样分化蛋白88(MyD88))mRNA的影响;方法:VORC(0.1 mL/kg、0.05 mL/kg、0.0025 mL/kg)灌胃(ig)给药5天,末次给药后1 h用LPS(1mg/kg)大鼠尾静脉注射制备急性肺炎模型,注射后6 h无菌取肺,实时荧光定量PCR方法检测,TLR2、TLR4和MYD88 mRNA的表达;结果:LPS尾静脉注射6 h,模型组TLR2、TLR4和MYD88 mRNA较空白组表达相对增高了6.41、6.50、4.89倍;VORC各剂量组TLR2、111R4和MYD88 mRNA均较模型组表达显著降低;结论:桂枝挥发油抗炎的药效学作用可能与抑制TLR2、TLR4和MYD88 mRNA表达,继而抑制其下游信号通路有关.
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| 关 键 词： | 桂枝挥发油 急性肺炎 Toll样受体2 Toll样受体4 髓样分化蛋白 |
Efect of Ramulus Cinnamomi Volatile Oils on Expression of Toll-like Receptor2 and Receptor4 and MYD88 on Acute Pneumonia Model Induced by LPS |
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| XU Shi-jun,SHEN Ying-jun,JIN Shen-rui,XIE Yu-huan.Efect of Ramulus Cinnamomi Volatile Oils on Expression of Toll-like Receptor2 and Receptor4 and MYD88 on Acute Pneumonia Model Induced by LPS[J].Journal of Chengdu University of Traditional Chinese Medicine,2008,31(3):32-34,37. |
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| Authors: | XU Shi-jun SHEN Ying-jun JIN Shen-rui XIE Yu-huan |
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| Institution: | XU Shi-jun,SHEN Ying-jun,JIN Shen-rui,XIE Yu-huan(Chendu university of TCM chengdu 610075) |
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| Abstract: | Objective: To investigate the influences of Ramulus Cinnamomi volatile oils to the acute pneumonia model by LPS on the expression of TLR2,TLR4 and MYD88 in rats acute pheumonia tiusse.Method:The model of acute pneumonia was made,After treatment 5 days by VORC,6 hours later,collected the lung tiusse in aseptic condition,extracted mRNA and measured the expression of TLR2,TLR4 and MYD88 by Real-time PCR,analyzed and evaluated VORC pharmacodynamic actions.Result:Compared with control group,the expression of TLR... |
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| Keywords: | Ramulus Cinnamomi volatile oils acute pneumonia TLR3 TLR4 MYD88 |
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