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In vitro angiogenic activity ofAloe vera gel on calf pulmonary artery endothelial (CPAE) cells |
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| Authors: | Myoung-Jin Lee Ok-Hee Lee Soo-Hong Yoon Seung-Ki Lee Myung-Hee Chung Young-In Park Chung-Ki Sung Jae-Sue Choi Kyu-Won Kim |
| Affiliation: | 1. Institute of Biotechnology, Yeungnam University, Taegu, Korea 2. Department of Molecular Biology, Pusan National University, 609-735, Pusan, Korea 3. College of Pharmacy, Catholic University of Taegu-Hyosung, Taegu, Korea 4. College of Pharmacy, Seoul National University, Seoul, Korea 5. College of Medicine, Seoul National University, Seoul, Korea 6. College of Natural Resources, Korea University, Seoul, Korea 7. College of Pharmacy, Chonman National University, Kwangju, Korea 8. Department of Food and Nutrition, Pukyong National University, Pusan, Korea |
| Abstract: | Angiogenic activity ofAloe vera gel was investigated byin vitro assay. We obtained the most active fraction from dichloromethane extract ofAloe vera gel by partitioning between hexane and 90% aqueous methanol. The most active fraction (F3) increased the proliferation of calf pulmonary artery endothelial (CPAE) cells. In addition, F3 fraction induced CPAE cells to invade type I collagen gel and form capillary-like tube throughin vitro angiogenesis assay, and increased the invasion of CPAE cells into matrigel throughin vitro invasion assay. Furthermore, the effect on the mRNA expression of proteolytic enzymes which are key participants in the regulation of extracellular matrix degradation was investigated by northern blot analysis. F3 fraction enhanced mRNA expression of urokinase-type plasminogen activator (u-PA), matrix metalloproteinase-2 (MMP-2), and membrane-type MMP (MT-MMP) in CPAE cells whereas the expression of plasminogen activator inhibitor-1 (PAL-1) mRNA was not changed. |
| Keywords: | Angiogenesis CPAE cells In vitro angiogenesis assay Urokinase-type plasminogen activator (u-PA) Matrix metalloproteinase (MMP) |
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