RacGAP1在胃癌中的表达与幽门螺杆菌L型感染的关系

目的 探讨胃癌组织中RacGAP1的表达情况,及其与幽门螺杆菌L型(Hp-L)感染的关系。方法 选取125例胃癌患者,根据有无Hp-L感染分为Hp-L感染阳性组(感染组)和Hp-L感染阴性组(未感染组),另选取胃镜检查为正常胃黏膜,且Hp-L感染阴性的体检者30例作为对照组,应用免疫组织化学法检测标本中RacGAP1蛋白的表达情况,应用逆转录-聚合酶链反应(RT-PCR)法检测各组中RacGAP1 mRNA的表达情况,并对胃癌患者进行为期5年的随访。结果 RacGAP1主要表达在胃癌组织细胞核中,感染组和未感染组RacGAP1阳性率显著高于对照组(P＜0.01),感染组RacGAP 1阳性率显著高于未感染组(P＜0.05)。RacGAP1 mRNA的表达组间存在显著差异:感染组＞未感染组＞对照组;RacGAP1蛋白在胃癌组织中的表达与瘤体大小、浸润深度、淋巴转移、TNM分期和Hp感染有关;感染组生存率63.6%(49/77),未感染组生存率83.3%(30/36)。未感染组的生存率显著高于感染组(P＜0.05)。结论 胃癌组织中RacGAP1表达显著升高,Hp感染可能导致RacGAP1表达的提高,且RacGAP1高表达的患者预后较差。

Relationship between RacGAP1 expression in gastric cancer and Helicobacter pylori L-type infection

Objective To explore the expression of RacGAP1 in gastric cancer tissue and its relationship with Helicobacter pylori L-type (Hp-L) infection.Methods A total of 125 cases of patients with gastric cancer were divided into Hp-L infection positive group (infection group) and Hp-L infection negative group (non-infection group), another 30 cases who had normal gastric mucosa and Hp-L non-infection were selected as control group. Immunohistochemical method was used to check the expression of Racgap1 protein in gastric cancer tissue. The Racgap1 mRNA expression was detected using RT-PCR method. All the patients were taken a period of five years of follow-up.Results RacGAP1 protein was expressed in nucleus mainly, and the positive rate of RacGAP1 expression in infection group and non-infection group were significantly higher than that of control group(P＜0.01), and the positive rate of RacGAP1 expression was significantly higher in infection group than in non-infection group(P＜0.05). RacGAP1 protein expression in gastric cancer tissues was associated with tumor size, invasion depth, lymph node metastasis, TNM staging and Hp infection. The survival rate of the non-infected group was significantly higher than that of the infected group (P<0.05).Conclusion RacGAP1 expression was significantly increased in gastric cancer tissues, Hp infection may be the reason leading to the increase of RacGAP1 expression, and the prognosis in patients with high expression of RacGAP1 was poor.