稳定期慢性阻塞性肺疾病患者非侵入性标记物研究

目的比较慢性阻塞性肺疾病（COPD）患者和健康受试者非侵入性标记物，评估其与吸烟的关系。方法选取在我院治疗的稳定期COPD戒烟患者52例（COPD EX组），稳定期COPD吸烟患者36例（COPD S组），在我院体检的健康的吸烟者23例（HE S组），健康的戒烟者23例（HE EX组）。比较所有受试者呼出气冷凝物（EBC）、痰液中前列腺素E2（PGE2）和异构前列腺素15 F2t isoprostane（15 F2t IsoP）的水平、呼出气一氧化氮（FeNO）、痰细胞计数和肺功能。结果与COPD S组比较，COPD EX组的FeNO值明显提高；与两健康组比较，两COPD组EBC中15 F2t IsoP水平明显升高（P＜0.05）；与同等健康状况的戒烟者比较，吸烟者尿液中15 F2t IsoP水平升高（P＜0.05）。结论不同类型炎症性标志物的联合检测分析可用于对COPD患者呼吸炎症的综合评估。

Non invasive markers in patients with stable chronic obstructive pulmonary disease

ObjectiveTo compare the non invasive markers between chronic obstructive pulmonary disease (COPD) patients and healthy subjects and to assess their relationship with smoking. MethodsThe subjects included 52 COPD ex smoking patients (COPD EX group，n=52) and 36 smoking patients (COPD S group，n=36) treated in our hospital， 23 healthy ex smokers (HE EX group，n=23) and 23 healthy smokers (HE S group，n=23) medically examined in our hospital. Prostaglandin E2 (PGE2)， and prostaglandin isomers 15 F2t isoprostane (15 F2t IsoP) concentrations in sputum， exhaled breath condensate (EBC)， fraction of exhaled nitric oxide， sputum cell counts and pulmonary function test were detected and compared. ResultsCompared with COPD S group， FeNO values in COPD EX group increased significantly (P<0.01); Compared with healthy group， the EBC 15 F2t IsoP concentrations in two groups with COPD increased significantly (P<0.05); Compared with smokers who had equal health status， the urine 15 F2t IsoP concentration in both smokers groups increased significantly (P<0.05).ConclusionThe combination of different types of inflammatory markers can be used for a comprehensive assessment of COPD respiratory inflammation.