同源盒蛋白1和叉头框蛋白C2在结肠癌中的表达及临床意义

目的探讨同源盒蛋白1（Six1）、叉头框蛋白C2（FOXC2）在结肠癌中的表达及其与结肠癌发生、发展和转移的关系。方法采用免疫组织化学法SP法检测70例结肠癌组织和30例癌旁正常组织中Six1、FOXC2的表达情况，分析两者在结肠癌发生发展中的相关性，及与肿瘤患者临床病理特征之间的关系。结果Six1、FOXC2在结肠癌中的阳性表达率分别为82.9%和74.3%，均明显高于癌旁正常组织中的表达(16.7%，20.0%，P<0.05)，且两者在结肠癌中的表达具有相关性（r=0.426，P<0.01）。Six1、FOXC2在结肠癌中的表达水平与肿瘤TNM分期、浸润深度、淋巴结转移密切相关（P<0.05)，而与患者年龄、性别、肿瘤大小、分化状态无关（P>0.05)。结论Six1、FOXC2高表达可能共同参与了结肠癌的发生发展过程并在结肠癌的侵袭转移过程中存在协同作用， 故二者的表达可能与结肠癌的不良预后具有密切联系。

Expression and clinical significance of Six1 and FOXC2 in colon cancer

ObjectiveTo investigate the expression of Six1 and FOXC2 in colon cancer and the relationship between their expression and the occurrence， development and metastasis of colon cancer. MethodsThe immunohistochemical method was used to determine the expression of Six1 and FOXC2 in 70 cases of colon cancer and 30 cases of adjacent normal tissues. The correlation between the two proteins and colon cancer process and the pathological features of colon cancer were explored. ResultsThe expression of Six1 and FOXC2 in colon cancer were significantly higher than those in adjacent normal tissues (82.9% vs 16.7% and 74.3% vs 20.0%，respectively，P<0.05）. Both proteins were positively correlated with colon adenocarcinoma（r=-0.426，P<0.01）. The expression of the two kinds of proteins were related with the TNM staging of tumor， depth of invasion and lymph node metastasis（P<0.05），no association was observed with patients' age， gender， tumor size and histological grading (P>0.05). ConclusionSix1 and FOXC2 were overexpressed in colon cancer. They may play important roles in carcinogenesis and progression of colon cancer and a positive correlation was shown between them， suggesting that the expression of two proteins may be closely associated with poor prognosis in colon cancer.