ELISA法检测急性血吸虫病患者血清HBV标志物时假阳性排除方法探讨

目的探讨ELISA法测定急性血吸虫病(下称急血)患者HBV标志物时假阳性的排除方法。方法将90例急血患者血清行抗干扰处理和未抗干扰处理,然后同时进行ELISA法检测HBV5项标志物,并且对该90例患者均进行HBVDNAPCR定量检测。结果进行抗干扰处理的标本HBV5项标志物(HBsAg、HBsAb、HBeAg、HBeAb、HBcAb)的阳性率依次分别为16.7%、38.9%、4.4%、27.8%、32.2%;未进行抗干扰处理的标本该5项标志物的阳性率依次分别为58.9%、67.8%、15.6%、43.3%、48.9%,HBV5项标志物的阳性率前者明显低于后者;HBVDNAPCR定量检测的阳性率为17.8%。且抗干扰处理后患者HBV5项标志物组合显示HBV现症感染的阳性率与HBVDNAPCR的阳性率相近。结论该血清标本抗干扰处理法是急血患者ELISA法测定HBV标志物时假阳性排除较有效的方法。

Exploring Study for Discriminating False Positive in HBV Markers Test by ELISA Among Patients With Co- infected Acute Schistosomiasis

Objective To explore the knack for discriminating the false positive HBV markers from the patients with co-infected acute schistosomiasis by routine ELISA method. Methods Ninety patients with acute schistosomiasis performing routine ELISA for HBV marker tests were divided into two groups, and their serum samples were processed by antijaming or without processing. Then the five ELISA reported HBV markers were checked accordingly, and also the serum samples were further confirmed by HBV DNA copies. Results The positive rates of the five HBV's markers (HBsAg, HBsAb, HBeAg, HBeAb, and HBcAb) from the antijaming treated group were 16.7%, 38.9%, 4.4%, 27.8%, and 32.2% vs. the untreated group of 58.9%, 67.8%, 15.6%, 43.3%, and 48.9% respectively. The positive rate for HBV DNA was 17.8% between both groups. Through the antijaming processing, the positive rate demonstrated by the five HBV markers became similar with that determined by HBV DNA testing. Conclusions The antijaming processing is the knack that can discriminate the negative serum from the false positive group, which the ELISA judged wrongly with patients infected with acute schistosomiasis.