| 大鼠骨髓间充质干细胞体外诱导分化为肝细胞 |
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| 引用本文： | 王忠琼,夏世萍,刘彦,钟晓琳,李昌平. 大鼠骨髓间充质干细胞体外诱导分化为肝细胞[J]. 泸州医学院学报, 2010, 33(1): 40-44 |
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| 作者姓名： | 王忠琼 夏世萍 刘彦 钟晓琳 李昌平 |
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| 作者单位： | 泸州医学院附属医院消化内科,四川泸州,646000 |
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| 摘 要： | 目的：研究HGF、aFGF及OSM诱导MSCs成为肝细胞的能力及其在分化中的作用。方法：用HGF、aFGF、OSM诱导分化MSCs后，用化学发光法、RT—PCR法及谷氨酸脱氢酶法检测细胞AFP、ALB及尿素的表达情况。结果：除对照组外其余各组细胞均有AFP表达（P〈0．05）；对照组及a＋O组无ALB、尿素的表达，其余各组出现ALB及尿素的表达（P〈0．05）。结论：HGF联合aFGF、OSM及aFGF能够诱导MSCs分化为表达AFP、ALB、尿素的肝细胞；aFGF与OSM在没有HGF存在时不能诱导MSCs向较为成熟的肝细胞的分化。
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| 关 键 词： | 骨髓间充质干细胞 肝细胞生长因子 酸性成纤维细胞生长因子 制瘤素M 肝细胞 |
DIFFERENTIATION OF MARROW BONE MESENCHYMAL STEM CELLS INTO HEPATOCYTES FROM RAT IN VITRO |
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| Wang Zhongqiong,Lv Muhan,Xia Shiping,Zhong Xiaolin,Li Changping. DIFFERENTIATION OF MARROW BONE MESENCHYMAL STEM CELLS INTO HEPATOCYTES FROM RAT IN VITRO[J]. Journal of Luzhou Medical College, 2010, 33(1): 40-44 |
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| Authors: | Wang Zhongqiong Lv Muhan Xia Shiping Zhong Xiaolin Li Changping |
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| Affiliation: | Wang Zhongqiong,Lv Muhan,Xia Shiping,Zhong Xiaolin,Li Changping Department of Gastroenterology,the Affiliated Hospital of Luzhou Medical College,Luzhou,646000,china |
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| Abstract: | Objectives:To study the potential of hepatocyte growth factor (HGF),acid fibroblast growth factor (aFGF) and oncostatin M (OSM) to induce MSCs differentiate into hepatocytes and their roles. Methods:MSCs were treated with HGF,aFGF and OSM. Detecting the expression of AFP,ALB and urea was performed by che-mi-luminescent technique,RT-PCR method and glutamic acid dehydrogenase technique. Results:AFP expression emerged in every group except control group (P<0.05). ALB and urea expression emerged in other groups... |
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| Keywords: | Bone marrow mesenchymal stem cells Hepatocyte growth factor Acidic fibroblast growth factors Oncostatin M Hepatocytes |
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