川芎嗪对人脐静脉内皮细胞株(Eahy926)氯化钴诱导缺氧后纤溶功能的影响

目的:建立人脐静脉内皮细胞株(Eahy926)缺氧细胞模型,探讨川芎嗪在缺氧条件下对血管内皮细胞纤溶功能的影响。方法:细胞分为对照组、缺氧组和川芎嗪(TMP)干预组,利用氯化钴(1.6mmol/L)模拟Eahy926细胞缺氧环境,建立缺氧模型,TMP干预组分别用0.08和0.16g/L的TMP干预。Hoechst法、CCK-8法检测各组细胞活力,RT-PCR观察各组细胞组织型纤溶酶原激活物(tissue-type plasminogen activator,t-PA)、尿激酶型纤溶酶原激活物(urokinase-type plasminogen activator,u-PA)、纤溶酶原激活物抑制物-1(plasminogen activator inhibit ortype-1,PAI-1)mRNA表达,双抗体夹心ELISA法检测各组细胞培养上清液中t-PA、u-PA、PAI-1含量。结果:与对照组相比,缺氧组及TMP干预组均出现细胞活力下降(P<0.01),但与缺氧组相比,TMP干预组细胞活力上升(P<0.01),且高浓度组较低浓度组上升明显(P<0.01);RT-PCR和ELISA结果显示:与对照组相比,...

Effect of tetramethylpyrazine on fibrinolytic function in human umbilical vein endothelial cell (Eahy926) treated by CoCl2

Objective:To establish hypoxia model with human umbilical vein endothelial cell line(Eahy926) and investigate the effect of tetramethylpyrazine(TMP) on Eahy926's fibrinolytic function with hypoxia.Methods:The cells were divided into three groups:control group,CoCl2-treated group(1.6 mmol/L) and CoCl2+TMP-treated groups(0.08 and 0.16 g/L,respectively).Hypoxia was mocked by treatment of CoCl2.Cell viability was evaluated by Hoechst staining and cell counting kit-8(CCK-8);the expression of t-PA,u-PA and PAI-1 ...