人胚鼻咽上皮细胞的传代培养和克隆生长

应用低血清和无血清培养基成功地获得了人胚鼻咽上皮细胞的传代培养和克隆生长,而无纤维细胞污染。植块培养细胞增殖旺盛,分裂相多见。早期生长晕内可见成群的纤毛细胞,以后逐渐呈现鳞状终未分化。上皮细胞可传代3～5次,存活期可达80天,组织块可重复植块培养3～4次,克隆形成率平均为12％。培养基中缺少氢化可的松,胰岛素时克隆形成率明显下降,血清浓度为1％时克隆形成率最高。此实验为鼻咽癌研究提供了一个新的模型。

SERIAL CULTIVATION AND CLONAL GROWTH OF HUMAN NASOPHARYNGEAL EPITHELIAL CELLS IN A LOW SERUM AND SERUM-FREE MEDIUM

In this paper, serial cultivation and clonal growth of normal human fetal nasophar-yngeal epithelial cells have been achieved in a low serum and serum-free medium, without fibroblast contamination. In the expiant culture the rapid multiplication and mitotic figures of epithelial cells were easily detected. Islands of ciliated epithelial cells were present in the early stage of outgrowth of expiant tissues. Gradually the epithelial cell under-went squamous differentiation. These nasopharyngeal epithelial cells could be passaged three to five times and be grown for periods of up to go days. Repeated transfer of ex-plant tissue to a new dish could initiate additional primary cultures for three to four times. Clonal growth rate was averagely 12 per cent in the serum-free medium containing all supplements and obviously reduced when deleting hydrocortisone and insulin in the medium. In the test of various serum concentrations, the clonal growth rate was highest when serum concentration was i per cent. Our experiments supply a new model for research of nasopharyngeal carcinoma.