| 采用9对引物检测假肥大型肌营养不良症的基因缺失 |
| |
| 引用本文： | 赵振军,叶贤坤,张义芳,王小会,牛丽霞. 采用9对引物检测假肥大型肌营养不良症的基因缺失[J]. 河北医药, 2001, 23(2): 98-99 |
| |
| 作者姓名： | 赵振军 叶贤坤 张义芳 王小会 牛丽霞 |
| |
| 作者单位： | 050091,石家庄,河北以岭医药研究院 |
| |
| 摘 要： | 目的：研究快速、准确检测假肥大型肌营养不良（DMD／BMD）基因缺失的方法。方法：采用9对引物的多重链式聚合酶反应（mPCR）检测169例DMD／BMD基因缺失。结果：169例患者中85例检测出基因缺失，总缺失率为50．3％。结论：9对引物的mPCR在DMD／BMD基因缺失诊断中具有快速、准确、经济、实用等特点，便于推广。
|
| 关 键 词： | 肌营养不良 基因缺失 mPCR 假肥大型肌营养不良反应 引物检测 |
| 修稿时间： | 2000-08-10 |
Detection of muscular dystrophy gene deletions by 9 pairs of primers |
| |
| ZHAO Zhenjun,YE Xiankun,ZHANG Yifang,et al.. Detection of muscular dystrophy gene deletions by 9 pairs of primers[J]. Hebei Medical Journal, 2001, 23(2): 98-99 |
| |
| Authors: | ZHAO Zhenjun YE Xiankun ZHANG Yifang et al. |
| |
| Affiliation: | ZHAO Zhenjun,YE Xiankun,ZHANG Yifang,et al.He Bei Yiling Medical Research Institute,Shijiazhuang 050091 |
| |
| Abstract: | Objective To investigate a quick and accurate method in detection of DMD/BMD gene deletions.Methods Detection of DMD/BMD gene deletions by mPCR of 9 pairs of primers was applied.Results 85 patients' gene was deleted in 169.The total rate of deletions was 50.3%.Conclusion mPCR of 9 pairs of primers was a quick accurate economic and practical method in detection of DMD/BMD gene deletions. |
| |
| Keywords: | muscular dystrophy gene deletion mPCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
