自制混合血浆建立血凝仪室内质控的可行性探讨

目的:探讨自制混合血浆建立血凝仪室内质控的方法。方法:收集凝血功能正常的病人新鲜混合血浆,分装于EP离心管后放置-20℃低温冰箱保存备用,每天从冰箱中取出2管,放置室温让其自然解冻,1管作为正常值血浆,另1管使用生理盐水准确稀释3倍作为异常值血浆,每天同时检测自制正常值与异常值血浆、配套质控品和临床标本的PT、APTT、Fib。结果:保存3个月内的自制混合血浆,PT、APTT、Fib检测结果与其靶值差异无统计学意义(P>0.05),保存第3个月的自制混合血浆与配套质控品的PT、APTT、Fib检测结果在L-J质控图上的走势一致,相关性良好(r>0.95)。结论:自制混合血浆低温保存,每天取出2管,1管可作为正常值质控血浆,另1管使用生理盐水准确稀释3倍可作为异常值质控血浆进行血凝仪室内质控。

FEASIBILITY DISCUSSION ON ESTABLISHING OF INTERNAL CONTROL OF BLOOD COAGULATION ANALYZER BY USING SELF-MADE MIXED BLOOD PLASMA

Objective: To explore the establishment of internal control of blood coagulation analyzer by using self-made mixed plasma.Methods: The fresh mixed blood plasma was collected from the patients with normal coagulation,divided into EP centrifuge tubes and then restored in low temperature refrigerator(-20 ℃) for conservation.Two tubes were removed from the refrigerator and placed at room temperature per day.After thawing unaffectedly,one was used as normal blood plasma,another was diluted with normal saline by three folds as abnormal blood plasma.PT,APTT,Fib were assayed simultaneously in the normal and abnormal self-made blood plasma,matching quality control substance and clinical specimens.Results: In three months,there was no statistically significance difference between the cryopreserved self-made mixed blood plasma and the raw self-made mixed blood plasma on the results of PT,APTT and Fib(P>0.05).In the third month,PT,APTT and Fib analysis showed the results of the cryopreserved self-made mixed plasma were consistent with those of the matching quality control substance in L-J quality control graph,they had good correlation(r>0.95).Conclusion: The cryopreserved self-made mixed plasma can be used as internal control on blood coagulation analyzer.Two tubes were removed per day,one can be used as normal control and the other diluted with normal saline by three folds can be used as abnormal control.