糖基化终产物对凝集素样氧化型低密度脂蛋白受体1表达的影响

目的 研究长期高血糖所致糖基化终产物对巨噬细胞凝集素样氧化型低密度脂蛋白受体1表达的影响.方法 U937细胞经佛波酯诱导分化,并将不同浓度或同一浓度糖基化终产物与诱导分化48 h后的U937细胞共同孵育,用Western Blotting法检测凝集素样氧化型低密度脂蛋白受体1蛋白的表达.同时应用ELISA法测定24例2型糖尿病患者及22例正常对照者血清可溶性氧化型低密度脂蛋白受体1的含量.结果 100、200和400 mg/L 糖基化终产物刺激后细胞表面凝集素样氧化型低密度脂蛋白受体1蛋白表达量分别是对照组的1.85、3.22和4.65倍(P<0.05);400 mg/L的糖基化终产物作用12、24、48 h后,U937巨噬细胞该受体蛋白表达量分别为0 h的2.85、3.89和4.3倍(P<0.05).糖尿病患者血清氧化型低密度脂蛋白受体1及糖基化终产物含量较正常对照者显著升高(P<0.01),两者呈正相关(P<0.001). 结论糖基化终产物可增加U937巨噬细胞凝集素样氧化型低密度脂蛋白受体1蛋白表达且呈浓度和时间依赖性.这可能与糖尿病患者加速泡沫细胞形成而易致动脉粥样硬化有关.

Effect of Advanced Glycation End Products on Expression of Lectin-Like Oxidized Low Density Lipoprotein Receptor-1

Aim To learn the effects of advanced glycation end products(AGE) on expression of lectin-like oxidized low density lipoprotein(LOX-1) protein in U937 macrophages and serum soluble LOX-1(sLOX-1). Methods U937 macrophages differentiating for 48 h were incubated with AGE in various concentrations and time.The expression of LOX-1 protein were detected by Western Blotting analysis.Serum sLOX-1 and AGE levels were detected by ELISA in 24 diabetes and 22 normal controls. Results After exposure of U937 macrophages to 100,200 or 400 mg/L AGE,the expression of LOX-1 protein was 1.85-,3.22-and 4.65-fold,as compared with that of control group(P><0.05),LOX-1 protein expression following 400 mg/L AGE for 12,24,48 h was 2.85-,3.89-and 4.3-fold compared with 0 h group(P><0.05).Serum sLOX-1 and AGE levels were significantly increased in diabetes group compared with that of control(P><0.01).They were positively correlated(P><0.001). Conclusion AGE could enhance the expression of LOX-1 protein in a time-and dose-dependent manner,which may play an important role in diabetic macroangiopathy through accelerating foam cell formation.