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| 肺癌组织中DNA修复基因ERCC1的表达与多环芳烃-DNA加合物的关系 | |
| 引用本文： | 王云南,吕嘉春,曾波航,宾晓农. 肺癌组织中DNA修复基因ERCC1的表达与多环芳烃-DNA加合物的关系[J]. 中国病理生理杂志, 2004, 20(7): 1153-1156 |
| 作者姓名： | 王云南 吕嘉春 曾波航 宾晓农 |
| 作者单位： | 1. 广州市胸科医院, 广东 广州 510095; 2. 广州医学院化学致癌研究所, 广东 广州 510182; 3. 广州医学院第二附属医院肿瘤科, 广东 广州 510260 |
| 基金项目： | 国家自然科学基金项目 (30 371196、30 2 0 0 2 35 )，广东省科技计划项目 (2 0 0 2C30 30 9、2 0 0 2B30 10 4 )，广东省医学科研项目 (B2 0 0 2 6 0 6、B2 0 0 10 75 )，广州市教委项目 (0 1-34) |
| 摘 要： | 目的:探讨核苷酸切除修复基因ERCC1在肺癌中的表达及其与多环芳烃(PAH)-DNA加合物的关系。方法: 用RT-PCR技术检测150例肺癌、120例癌旁肺组织、40例肺良性病变和40例正常肺组织中ERCC1基因mRNA的表达;用免疫法检测PAH-DNA加合物;分析有关暴露因素对修复基因ERCC1的表达和PAH-DNA加合物的影响,并探讨ERCC1与PAH-DNA加合物的关系。结果:30.7%(46/150)的肺癌组织和2.5%(1/40)的正常肺组织存在ERCC1基因的表达低下;吸烟可抑制ERCC1基因的表达。肺组织中PAH-DNA加合物的水平与ERCC1基因表达呈显著负相关,Spearman相关系数为-0.648,P<0.01。结论:ERCC1是参与修复DNA加合物等损伤的一个重要的核苷酸切除修复基因,其表达低下在肺癌发生中起着重要作用。 |
| 关 键 词： | 核苷酸类 肺肿瘤 DNA加合物 基因 ERCC1 |
| 文章编号： | 1000-4718(2004)07-1153-04 |
| 收稿时间： | 2003-12-26 |
| 修稿时间： | 2004-04-01 |
Expression of DNA repair gene ERCC1 and its relationship with PAH- DNA adducts in lung cancer tissues |
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| WANG Yun-nan,LV Jia-chun,ZENG Bo-hang,BIN Xiao-nong. Expression of DNA repair gene ERCC1 and its relationship with PAH- DNA adducts in lung cancer tissues[J]. Chinese Journal of Pathophysiology, 2004, 20(7): 1153-1156 | |
| Authors: | WANG Yun-nan LV Jia-chun ZENG Bo-hang BIN Xiao-nong |
| Affiliation: | 1. Guangzhuo Chest Hospital, Guangzhou 510095, China; 2. The Institute for Chemical Carcinogenesis, Guangzhou Medical College, Guangzhou 510182, China; 3. Department of Tumor, Second Affiliated Hospital, Guangzhou Medical College, Guangzhou 510260, China |
| Abstract: | AIM: To investigate the expression of nucleotide excision repair gene ERCC1 and its relationship with PAH (polycyclic aromatic hydrocarbons)-DNA adducts in lung cancer tissues. METHODS: ERCC1 mRNA expression and the PAH-induced DNA adducts were detected in 150 lung cancer tissues, 120 adjacent lung tissues without cancer cells, 40 benign lung lesions and 40 normal lung tissues. The effects of some exposure factors on the expression of ERCC1 gene and the connection between ERCC1 and PAH-DNA adduct was analyzed. RESULTS: Reduced expression levels of ERCC1 were observed in 46 of 150 (30.7%) lung cancer specimens and 1 of 40 (2.5%) normal lung tissues. Smoking may suppress the expression of ERCC1 gene. The level of PAH-DNA adduct was negatively correlated with the expression of ERCC1 gene, the Spearman coefficient was -0.648, P<0.01. CONCLUSION: ERCC1 is an important nucleotide excision repair gene and may participate in the repair of DNA damage, such as PAH-DNA adduct. Low expression of ERCC1 may play an important role in the development of human lung cancer. [ |
| Keywords: | Nucleotides Lung neoplasms DNA adducts Gene ERCC1 |
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