| ELISA法检测可溶性人类白细胞抗原-Ⅰ及广东人参考值的测定 |
| |
| 引用本文： | 吴涛,兰炯采,张泓,王从容,毛高才. ELISA法检测可溶性人类白细胞抗原-Ⅰ及广东人参考值的测定[J]. 南方医科大学学报, 2002, 22(10): 931-933 |
| |
| 作者姓名： | 吴涛 兰炯采 张泓 王从容 毛高才 |
| |
| 作者单位： | 第一军医大学南方医院输血科,广东广州,510515 |
| |
| 摘 要： | 目的探讨可溶性人类白细胞抗原-Ⅰ(sHLA-Ⅰ)的定量检测技术,并用于检测广东地区人群的参考值。方法以W6/32包被酶标板,捕捉样品中sHLA-Ⅰ,β2微球蛋白抗体为一抗,再加酶标二抗及底物显色。根据不同浓度标准sHLA-Ⅰ显色后Dλ值的标准曲线,检测60 例正常广东人的sHLA-Ⅰ含量。结果sHLA-Ⅰ最低检测限为2.84 ng/ml,批内变异系数为5.80%,批间变异系数为9.00%,回收率为98.57%~100.15%。广东人sHLA-Ⅰ正常值为(699.54±360.10)ng/ml。结论ELISA法检测sHLA-Ⅰ具有灵敏度高、特异性强、稳定性好等优点。
|
| 关 键 词： | 可溶性人类白细胞抗原 酶联免疫吸附试验 定量检测 参考值 |
| 文章编号： | 10000-2588(2002)10-0931-03 |
| 修稿时间： | 2002-02-04 |
Detection of soluble HLA class Ⅰ antigens by ELISA and determination of its reference value in population of Guangdong province |
| |
| WU Tao,LAN Jiong-cai,ZHANG Hong,WANG Cong-rong,MAO Gao-cai. Detection of soluble HLA class Ⅰ antigens by ELISA and determination of its reference value in population of Guangdong province[J]. Journal of Southern Medical University, 2002, 22(10): 931-933 |
| |
| Authors: | WU Tao LAN Jiong-cai ZHANG Hong WANG Cong-rong MAO Gao-cai |
| |
| Affiliation: | WU Tao,LAN Jiong-cai,ZHANG Hong,WANG Cong-rong,MAO Gao-cai Department of Transfusion,Nanfang Hospital,First Military Medical University,Guangzhou 510515,China |
| |
| Abstract: | |
| |
| Keywords: | soluble human leukocyte antigens enzyme-linked immunosorbent assay quantification assay reference values |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
