High efficiency transformation of Kluyveromyces marxianus by a replicative plasmid |
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Authors: | François Iborra |
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Institution: | (1) Laboratoire de Biologie et Génetique Moléculaire, Institut de Génétique et Microbiologie Bat 400, Université Paris 11, F-91405 Orsay Cedex, France |
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Abstract: | Kluyveromyces marxianus can be transformed with an efficiency of 105 transformants/ g of DNA by a replicative plasmid using electroporation. In order to obtain this efficiency, we isolated ura- mutants cells which can be complemented by the URA3 gene from Saccharomyces cerevisiae. The URA3 gene and KARS2, a replicative origin from Kluyveromyces lactis which functions in K. marxianus, were ligated together in a plasmid which can be used as a vector to transform this strain. |
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Keywords: | Kluyveromyces marxianus Transformation Replicative vector |
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