骨髓间充质干细胞与心肌细胞共培养诱导分化为心肌样细胞

本研究探讨间充质干细胞（MSC）分化为心肌细胞（CM）的能力。对大鼠源MSC进行标记并将大鼠源MSC与初生大鼠CM直接接触共培养5—7天，诱导MSC向CM分化。用流式细胞仪检测细胞表面抗原，免疫荧光法检测标记细胞的心肌特异性标记物肌球蛋白和肌钙蛋白T表达。结果显示：体外培养的MSC表达CD90、CD44、CD105、CD54，不表达CD34、CD45、CD31等造血细胞、内皮细胞相关抗原。MSC与CM直接接触共培养后可分化为心肌样细胞，表达心肌特异蛋白肌钙蛋白T和肌球蛋白，CM与MSC比例为4：1时，MSC分化为心肌样细胞的比例最高。结论：MSC在与CM直接接触共培养条件下可被诱导分化为心肌样细胞，两种细胞按4：1比例混合培养后MSC分化为心肌细胞的效果最明显：

Differentiation of Bone Marrow Derived from Mesenchymal Stem Cells into Cardiomyocyte-like Cells Induced by Co-culture with Rat Myocardial Cells

The study was purposed to investigate the differentiation ability of mesenchymal stem cells(MSCs) into myocardial cells in vitro.Rat bone marrow-derived MSCs were labeled and co-cultured with neonatal rat cardiomyocytes(CM) for 5-7 days.The expression of cell surface antigenes was detected by flow cytometry,and the expression of muscle-specific marker myosin and troponin T in labeled cells was detected by immunofluorescence.The results showed that in vitro cultured MSCs expressed CD90,CD44,CD105,CD54,not expressed CD34,CD45,CD31.After co-cultured with neonatal rat CM,labeled MSCs differentiated into cardiomyocyte-like cells expressing myosin and troponin T.It is concluded that MSCs can differentiate into cardiomyocyte-like cells when co-cultured with neonatal myocardial cells in vitro.In co-culture of two kind of cells in ratio of four to one showed obvious efficacy differentiating MSCs into CMs.