miR-152调控糖尿病心肌病心肌成纤维细胞增殖的作用

目的探究miR-152调控糖尿病心肌病心肌成纤维细胞(CFs)增殖的作用。方法应用链脲佐菌素(STZ)构建糖尿病大鼠模型,应用高糖(33.3 mmol·L-1)诱导CFs增殖模型。多聚甲醛固定心肌组织后,行HE和Masson染色;Western blot检测心肌组织和CFs中α-SMA、CollagenⅠ的蛋白表达;qPCR检测心肌组织和CFs中miR-152的表达;MTT检测miR-152对细胞增殖活性的影响。结果HE和Masson染色显示,糖尿病组大鼠心肌胶原含量明显增多,细胞排列紊乱;在高糖模型中,α-SMA和CollagenⅠ表达增加,而miR-152表达降低;乳鼠CFs转染miR-152模拟物后,α-SMA和CollagenⅠ表达降低,同时CFs增殖活性减弱。结论miR-152可能在糖尿病心肌病CFs增殖中起重要作用,提示其可能改善糖尿病心肌病。

Effect of miR-152 on proliferation of myocardial fibroblasts in diabetic cardiomyopathy

Aim To explore the effect of miR-152 on proliferation of cardiac fibroblasts(CFs)in diabetic cardiomyopathy.Methods Diabetic cardiomyopathy model was established in SD rats by STZ injection,and CFs proliferation model was established by high glucose(33.3 mmol·L-1).HE and Masson staining were performed in paraformaldehyde fixed myocardium of rats.Western blot determinedα-SMA and collagenⅠprotein expression.qPCR detected gene expression of miR-152.MTT assay analyzed the proliferation of cells.Results HE and Masson staining showed the higher level of myocardial collagen in diabetic cardiomyopathy model.Furthermore,the myocardial myocytes lined up in disorder.Western blot showed that the expressions ofα-SMA and collagen I were up-regulated in the diabetes mellitus(DM)group,while the expression of miR-152 was down-regulated.The result of the in vitro experiment showed thatα-SMA and collagenⅠexpressions were down-regulated after transfected miR-152 mimics.The proliferation of CFs was also down-regulated after transfected miR-152 mimics.Conclusions miR-152 plays an important role in the proliferation of CFs and may ameliorate diabetic cardiomyopathy.