基于尿液代谢组学研究豆豉抗骨质疏松作用机制

目的探究豆豉对去卵巢骨质疏松大鼠骨代谢的影响及作用机制。方法将3月龄SPF级SD♀大鼠随机分为假手术组、模型组、雌二醇组及豆豉组,每组8只。手术后1周,通过灌胃将相应药物给予8周。8周后,测量每组的骨矿物质密度(BMD)和子宫指数,利用尿液代谢组学方法鉴定切除卵巢骨质疏松大鼠的生物标记物。结果(1)豆豉可明显增加模型组去卵巢大鼠股骨的BMD、子宫指数,明显降低模型组去卵巢大鼠体质量。(2)确定了与绝经后骨质疏松症密切相关的23个生物标记物及9条代谢通路。豆豉通过调节多种代谢途径,例如肌醇磷酸代谢、初级胆汁酸生物合成代谢和嘌呤代谢,可明显对12个生物标记物产生回调作用。结论豆豉能够有效干预绝经后骨质疏松症的发生和发展,该研究为预防绝经后骨质疏松症药物的研究与开发提供科学的依据。

Study on mechanism of anti-osteoporosis of soybean meal based on urine metabolomics

Aim To explore the effect of soybean meal on bone metabolism in ovariectomized osteoporosis ratsandits mechanism.Methods Three-month-old SPF-class SD female rats were randomly divided into sham operation group,model group,estradiol group and soybean meal administration group,with 8 rats in each group.One week after the operation,the corresponding drug was administered by gavage for 8 weeks.After 8 weeks,the bone mineral density(BMD)and uterus index of each group were measured.Biomarkers for ovariectomized osteoporosis rats were identified by urinary metabolomics.Results(1)Soybean meal could significantly increase BMD and uterine index of femur in ovariectomized rats in model group,and significantly reduce the weight of ovariectomized ratsin model group.(2)23 biomarkers and 9 metabolic pathways closely related to postmenopausal osteoporosiswere identified.Soybean meal could significantly exert a callback effect on 12 biomarkers by regulating various metabolic pathways,such as steroid hormone biosynthesis metabolism,inositol phosphate metabolism,primary bile acid biosynthesis metabolism and purine metabolism.Conclusions Soybean meal can effectively interfere with the occurrence and development of postmenopausal osteoporosis.This study provides scientific data for the prevention and treatment of postmenopausal osteoporosis drugs.