褪黑素对PDGF诱导的肝星状细胞中JAK2/STAT3信号通路的影响

目的探讨褪黑素对血小板衍生生长因子(PDGF)诱导的肝星状细胞-T6(HSC-T6)JAK2/STAT3信号通路的影响。方法将HSC-T6细胞分为6组:对照组、模型组、实验组(褪黑素1 nmol·L^-1、1μmol·L^-1、0.1 mmol·L^-1)、抑制剂组(AG490为JAK2/STAT3通路的抑制剂),MTT实验检测褪黑素对PDGF激活的HSC-T6细胞增殖的影响;免疫组化和Western blot检测褪黑素HSC-T6细胞中p-JAK2、p-STAT3蛋白的表达。结果与对照组比较,PDGF能明显激活HSC-T6细胞增殖,明显上调HSC-T6细胞中p-JAK2、p-STAT3的表达;与模型组比较,褪黑素和抑制剂均可抑制PDGF激活的HSC-T6细胞增殖,显著下调p-JAK2、p-STAT3的表达。结论褪黑素可抑制PDGF诱导的HSC-T6的活化与增殖,其机制可能与抑制JAK2/STAT3信号通路有关。

Effects of melatonin on PDGF-induced JAK2/STAT3 signaling pathway in hepatic stellate cells

Aim To investigate the effect of melatonin(Mel)on platelet derived growth factor(PDGF)-in-duced JAK2/STAT3 signaling pathway in hepatic stel-late cells(HSC-T6).Methods The HSC-T6 cells were divided into six groups:control group,model group,experimental group(melatonin 1 nmol·L^-1,1μmol·L^-1,0.1 mmol·L^-1)and inhibitor(AG490)group.MTT assay was used to detect the effect of me-latonin on the proliferation of PDGF-activated HSC-T6 cells.The expressions of p-JAK2 and p-STAT3 were detected by immunohistochemistry and Western blot.Results Compared with control group,PDGF could significantly activate the proliferation of HSC-T6 cells,increase the expression of p-JAK2 and p-STAT3 in HSC-T6 cells;compared with model group,melatonin and inhibitor(AG490)could inhibit the proliferation of PDGF-activated HSC-T6 cells,and significantly down-regulate the expression of p-JAK2 and p-STAT3.Conclusions Melatonin can inhibit the activation and proliferation of PDGF-induced HSC-T6 cells,and its mechanism may be related to the inhibition of JAK2/STAT3 signaling pathway.